LOCUS       sequence-157784-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter containing 13 copies of the p53-binding 
            consensus sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, 
            Lin D, Mercer WE, Kinzler KW, Vogelstein B
  TITLE     WAF1, a potential mediator of p53 tumor suppression.
  JOURNAL   Cell. 1993 Nov 19. 75(4):817-25.
  PUBMED    8242752
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1993 
            Nov 19. 75(4):817-25."
COMMENT     Sequence Label: PG13-luc (wt p53 binding sites)
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(490..506)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(490..506)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(626..644)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ataagcttga tgccaggcaa gtccaggcag gccaggcaag tccaggcagg ccaggcaagt
       61 ccaggcaggc caggcaagtc caggcaggcc aggcaagtcc aggcaggcca ggcaagtcca
      121 ggcaggccag gcaagtccag gcaggccagg caagtccagg caggccaggc aagtccaggc
      181 aggccaggca agtccaggca gggccaggca agtccaggca ggccaggcaa gtccaggcag
      241 gccaggcaag tccaggcagg atcgaattcg atctgttttt tttagtatta agcagaggcc
      301 gggggcccct ggcctccgct tactctggag aaaaagaaga gaggcattcc agaggcaact
      361 tgtcaaaaca ggactggcgc cttggaggcg ctgtggggcc acccaaattg atataattaa
      421 gccccaaccg cctcttcccg cctcatttca gcctcaccac cctcgacaag cttatcgata
      481 ccgtcggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc gagcttggca
      541 ttccggtact gttggtaaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt
      601 ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
      661 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc
      721 ggaatacttc ga
//
