LOCUS       sequence-157794-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian 
            cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 7 - CMVp-Cas9-3xNLS-HSVpA
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(166..186)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(193..213)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(238..285)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
       61 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg
      121 cttggtcggt catttcgaac cccagagtcc cgcttacccg ggtatcacct tcctcttttt
      181 cttggggtct tcaaccttcc ttttcttctt tggggatccg gcgccgctgc cgctagcttt
      241 ctttttctta gcttgaccag ctttcttagt agcagcagga cgcttgtcgc ctcccagctg
      301 agacaggtcg atccgtgtct cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc
      361 cagcacctct ttggtgctgg tgtacctctt ccggtcgatg gtggtgtcaa agtacttgaa
      421 ggcggcaggg gctcccagat tggtcagggt aaacaggtgg atgatattct cggcctgctc
      481 tctgatgggc ttatcccggt gcttgttgta ggcggacagc actttgtcca gattagcgtc
      541 ggccaggatc actctcttgg agaactcgct gatctgctcg atgatctcgt ccaggtagtg
      601 cttgtgctgt tccacaaaca gctgtttctg ctcattatcc tcgggggagc ccttcagctt
      661 ctcatagtgg ctggccaggt acaggaagtt cacatatttg gagggcaggg ccagttcgtt
      721 tcccttctgc agttcgccgg cagaggccag cattctcttc cggccgtttt ccagctcgaa
      781 cagggagtac ttaggcagct tgatgatcag gtcctttttc acttctttgt agcccttggc
      841 ttccagaaag tcgatgggat tcttctcgaa gctgcttctt tccatgatgg tgatccccag
      901 cagctctttc acactcttca gtttcttgga ct
//
