LOCUS       sequence-157797-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 30nt stgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 9 - U6p_30nt_stgRNA
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(85..141)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
       61 cgtcgcggtg agttcaggct ttttaggtcc agggttctcc tccacgtctc cagcctgctt
      121 cagcaggctg aagttagtag ctccgcttcc cttgtacagc tcgtccatgc cgagagtgat
      181 cccggcggcg gtgcggaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      241 gctcagcacg gactgggtgc tcaggtagtg gctgtcgggc agcagcacgg ggccgtcgcc
      301 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct ccacgttgtg
      361 gcggatcttg aagttcacct tgatgccgtt cttctgcttg acggccatga tatagatgtt
      421 gtggctgttg aagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      481 gacgcccttc agctcgatgc ggttcactag ggtgtcgccc tcgaacttca cctcggcgcg
      541 ggtcttgtag gtgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
      601 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcactg
      661 cacgccgtgg ctcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
      721 gcagatgaac ttcagggtca gcttgccgtt ggtggcatcg ccctcgccct cgcccctcac
      781 gctgaacttg tggccgttta cgtcgccgtc
//
