LOCUS       sequence-157803-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9N in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pAAV-SMVP-Cas9N
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagccgctag cgctaccggt cgccaccatg gccccaaaga agaagcggaa ggtcggtatc
       61 cacggagtcc cagcagccga caagaagtac tccattgggc tcgatatcgg cacaaacagc
      121 gtcggctggg ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt caaagttctg
      181 ggcaataccg atcgccacag cataaagaag aacctcattg gcgccctcct gttcgactcc
      241 ggggaaacgg ccgaagccac gcggctcaaa agaacagcac ggcgcagata tacccgcaga
      301 aagaatcgga tctgctacct gcaggagatc tttagtaatg agatggctaa ggtggatgac
      361 tctttcttcc ataggctgga ggagtccttt ttggtggagg aggataaaaa gcacgagcgc
      421 cacccaatct ttggcaatat cgtggacgag gtggcgtacc atgaaaagta cccaaccata
      481 tatcatctga ggaagaagct tgtagacagt actgataagg ctgacttgcg gttgatctat
      541 ctcgcgctgg cgcatatgat caaatttcgg ggacacttcc tcatcgaggg ggacctgaac
      601 ccagacaaca gcgatgtcga caaactcttt atccaactgg ttcagactta caatcagctt
      661 ttcgaagaga acccgatcaa cgcatccgga gttgacgcca aagcaatcct gagcgctagg
      721 ctgtccaaat cccggcggct cgaaaacctc atcgcacagc tccctgg
//
