LOCUS       sequence-157804-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral construct for building stable cell lines expressing Cas9 
            via puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(458..514)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(533..553)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(560..580)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(605..652)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
       61 ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
      121 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
      181 gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg
      241 ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga
      301 ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccgggtcg acggtgtggc
      361 gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggcccgg gggacgtcgt
      421 cgcgggtggc gaggcgcacc gtgggcttgt actcggtagg tccagggttc tcctccacgt
      481 ctccagcctg cttcagcagg ctgaagttag tagctccgct tccagcgctt atcaccttcc
      541 tctttttctt ggggtcttca accttccttt tcttctttgg ggatccggcg ccgctgccgc
      601 tagctttctt tttcttagct tgaccagctt tcttagtagc agcaggacgc ttgtcgcctc
      661 ccagctgaga caggtcgatc cgtgtctcgt acaggccggt gatgctctgg tggatcaggg
      721 tggcgtccag cacctctttg gtgctggtgt acctcttccg gtcgatggtg gtgtcaaagt
      781 acttgaaggc ggcaggggct cccagattgg tcagggtaaa caggtggatg atattctcgg
      841 cctgctctct gatgggctta tcccggtgct tgttgtaggc ggacagcact ttgtccagat
      901 tagcgtcggc caggatcact ct
//
