LOCUS       sequence-157811-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mutation based toggling reporter (MTBR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 13 - CMVp_U6p_27nt1_GFP(+3)_RFP(+2)
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             137..190
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(333..351)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     601..620
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agtaaggccg gccagccacg gcttcccccc tgaggtggcc gctcaggacg atggcaccct
       61 gcccatgagc tgcgcccagg agagcggcat ggacaggcac cccgccgctt gcgccagcgc
      121 taggatcaac gtgggtgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
      181 tccgggccct gtgagcaagg gcgaggagga taactccgcc atcatcaagg agttcctgcg
      241 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
      301 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
      361 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
      421 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
      481 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctctct
      541 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
      601 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
      661 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
      721 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
      781 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
      841 gtacgaacgc gccgag
//
