LOCUS       sequence-157815-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expressing Dox and IPTG inducible stgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Perli SD, Cui CH, Lu TK
  TITLE     Continuous genetic recording with self-targeting CRISPR-Cas in human
            cells.
  JOURNAL   Science. 2016 Aug 18. pii: aag0511.
  PUBMED    27540006
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 18. pii: aag0511."
COMMENT     Sequence Label: Construct 31 -
            1xTetO_H1p_20nt2_stgRNA_3xLacO_...
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..419)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="AKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDV
                     TLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGRE
                     FALRDPAGNCVHFVAEEQD"
     CDS             complement(420..476)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ttgtaatcca gaggttgatt atcgataagc ttgatatcga attcttatca gtcctgctcc
       61 tcggccacga agtgcacgca gttgccggcc gggtcgcgca gggcgaactc ccgcccccac
      121 ggctgctcgc cgatctcggt catggccggc ccggaggcgt cccggaagtt cgtggacacg
      181 acctccgacc actcggcgta cagctcgtcc aggccgcgca cccacaccca ggccagggtg
      241 ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc
      301 acaccggcga agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc ggtccagaac
      361 tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt caacttggcg
      421 ggtccggggt tctcctccac atcaccggct tgtttaagaa gagagaaatt ggttgcacca
      481 gagcccttgt acagctcgtc catgccgaga gtgatcccgg cggcggtgcg gaactccagc
      541 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gcacggactg ggtgctcagg
      601 tagtggctgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
      661 tcggcgagct gcacgctgcc gtcctccacg ttgtggcgga tcttgaagtt caccttgatg
      721 ccgttcttct gcttgacggc catgatatag atgttgtggc tgttgaagtt gtactccagc
      781 ttgtgcccca ggatgttgcc gtcctccttg aagtcgacgc ccttcagctc gatgcggttc
      841 actagggtgt cgcc
//
