LOCUS       sequence-157822-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Smad4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Moren A, Itoh S, Moustakas A, Dijke P, Heldin CH
  TITLE     Functional consequences of tumorigenic missense mutations in the 
            amino-terminal domain of Smad4.
  JOURNAL   Oncogene. 2000 Sep 7;19(38):4396-404.
  PUBMED    10980615
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene.";
            date: "2000-09-7"; volume: "19(38)"; pages: "4396-404"
COMMENT     Sequence Label: pcDNA3-Flag-Smad4
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttggta ccgagctcgg atccaccgcc atggactaca aggacgatga tgacaagggc
      121 gaattcgaga atatgtctat tacgaataca ccaacaagta atgatgcctg tctgagcatt
      181 gtgcatagtt tgatgtgcca tagacaaggt ggagagagtg aaacatttgc aaaaagagca
      241 attgaaagtt tggtaaagaa gctgaaggag aaaaaagatg aattggattc tttaataaca
      301 gctataacta caaatggagc tcatcctagt aaatgtgtta ccatacagag aacattggat
      361 gggaggcttc aggtggctgg tcggaaagga tttcctcatg tgatctatgc ccgtctctgg
      421 aggtggcctg atcttcacaa aaatgaacta aaacatgtta aatattgtca gtatgcgttt
      481 gacttaaaat gtgatagtgt ctgtgtgaat ccatatcact acgaacgagt tgtatcacct
      541 ggaattgatc tctcaggatt aacactgcag agtaatgctc catcaagtat gatggtgaag
      601 gatgaatatg tgcatgactt tgagggacag ccatcgttgt ccactgaagg acattcaatt
      661 caaaccatcc agcatccacc aagtaatcgt gcatcgacag agacatacag caccccagct
      721 ctgttagccc catctgagtc taatgctacc agcactgcca actttcccaa cattcctgtg
      781 gcttccacaa gtcagcctgc cagtatactg gggggcagcc atagtgaagg actgttgcag
      841 atagcatcag g
//
