LOCUS       sequence-157824-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Smad4 L43S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Moren A, Itoh S, Moustakas A, Dijke P, Heldin CH
  TITLE     Functional consequences of tumorigenic missense mutations in the 
            amino-terminal domain of Smad4.
  JOURNAL   Oncogene. 2000 Sep 7;19(38):4396-404.
  PUBMED    10980615
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene.";
            date: "2000-09-7"; volume: "19(38)"; pages: "4396-404"
COMMENT     Sequence Label: pcDNA3-Flag-Smad4 L43S
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             87..110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttg
       61 gtaccgagct cggatccacc gccatggact acaaggacga tgatgacaag ggcgaattcg
      121 agaatatgtc tattacgaat acaccaacaa gtaatgatgc ctgtctgagc attgtgcata
      181 gtttgatgtg ccatagacaa ggtggagaga gtgaaacatt tgcaaaaaga gcaattgaaa
      241 gttcggtaaa gaagctgaag gagaaaaaag atgaattgga ttctttaata acagctataa
      301 ctacaaatgg agctcatcct agtaaatgtg ttaccataca gagaacattg gatgggaggc
      361 ttcaggtggc tggtcggaaa ggatttcctc atgtgatcta tgcccgtctc tggaggtggc
      421 ctgatcttca caaaaatgaa ctaaaacatg ttaaatattg tcagtatgcg tttgacttaa
      481 aatgtgatag tgtctgtgtg aatccatatc actacgaacg agttgtatca cctggaattg
      541 atctctcagg attaacactg cagagtaatg ctccatcaag tatgatggtg aaggatgaat
      601 atgtgcatga ctttgaggga cagccatcgt tgtccactga aggacattca attcaaacca
      661 tccagcatcc accaagtaat cgtgcatcga cagagacata cagcacccca gctctgttag
      721 ccccatctga gtctaatgct accagcactg ccaactttcc caacattcct gtggcttcca
      781 caagtcagcc tgccagtata ctggggggca gccatagtga
//
