LOCUS       sequence-157826-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Smad4 R100T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Moren A, Itoh S, Moustakas A, Dijke P, Heldin CH
  TITLE     Functional consequences of tumorigenic missense mutations in the 
            amino-terminal domain of Smad4.
  JOURNAL   Oncogene. 2000 Sep 7;19(38):4396-404.
  PUBMED    10980615
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene.";
            date: "2000-09-7"; volume: "19(38)"; pages: "4396-404"
COMMENT     Sequence Label: pcDNA3-Flag-Smad4 R100T
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        29..47
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acccactgtc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
       61 ggtaccgagc tcggatccac cgccatggac tacaaggacg atgatgacaa gggcgaattc
      121 gagaatatgt ctattacgaa tacaccaaca agtaatgatg cctgtctgag cattgtgcat
      181 agtttgatgt gccatagaca aggtggagag agtgaaacat ttgcaaaaag agcaattgaa
      241 agtttggtaa agaagctgaa ggagaaaaaa gatgaattgg attctttaat aacagctata
      301 actacaaatg gagctcatcc tagtaaatgt gttaccatac agagaacatt ggatgggagg
      361 cttcaggtgg ctggtcggaa aggatttcct catgtgatct atgcccgtct ctggacgtgg
      421 cctgatcttc acaaaaatga actaaaacat gttaaatatt gtcagtatgc gtttgactta
      481 aaatgtgata gtgtctgtgt gaatccatat cactacgaac gagttgtatc acctggaatt
      541 gatctctcag gattaacact gcagagtaat gctccatcaa gtatgatggt gaaggatgaa
      601 tatgtgcatg actttgaggg acagccatcg ttgtccactg aaggacattc aattcaaacc
      661 atccagcatc caccaagtaa tcgtgcatcg acagagacat acagcacccc agctctgtta
      721 gccccatctg agtctaatgc taccagcact gccaactttc ccaacattcc tgtggcttcc
      781 acaagtcagc ctgccagtat actggggggc agccatagtg aaggactg
//
