LOCUS       sequence-157830-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of EPN-01* in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pET29b-EPN-01*
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(196..225)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtgctcgagt cactgagaag acgggtcgtt
       61 accgaacaga gaacgcagag aggtcagcgg gtacagttct ttgtcgatcg gctcctgttt
      121 ttgcggtggc gtggtggttt caacaccaga acggaaagat tcttccggcg gtgcggtcgg
      181 ttccggacga gactgcagat cttcttcgct aatcagtttc tgttcggtgc agccacgaat
      241 cttctcaacg aacgccttgg ccttttccgc cacttccacc ggggtgcctt tcactaacgc
      301 gctgcccacg cccacggcca gcacgcccgc tttgaaccat tcgcacacat tgtccagatt
      361 aacaccgccg gttggcacaa atttcacatt cggaaacgga cctttcatcg ctttaacaaa
      421 ctgcggaccc accacctcac ccggaaacag ttttaagatg gtatggccca gtttcatggc
      481 tttcaccagt tcggtcgggg tcataacgcc tggcatatag aacacaccct tctccttgca
      541 aaattggcta atttcctcgt ccaggtgcgg actcacaatg aattcggcgc cactttccac
      601 cgctttacgg cactgctcca cgctggtcac ggtgcccgca ccaataatcg cgcccatttc
      661 cttcagaaag ctcagttcct taatcacggt atccgcatcc ggcacggtaa aggtaatttc
      721 aatcagatga acgccgccca ggaaaaccgc cagcgctttc tttttcgctt cttccacact
      781 attggcacgc agcacggcaa cgatcttgtg tttcttga
//
