LOCUS       sequence-157833-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Smad7 N terminal domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  TITLE     Moustakas plasmids
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Smad7-N
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        29..47
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acccactgtc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
       61 ggtaccgagc tcggatccac cgccatggac tacaaggacg atgatgacaa gggcgaattc
      121 gggttcagga ccaaacgatc tgcgctcgtc cggcgtctct ggaggagccg tgcgcccggc
      181 ggcgaggacg aggaggaggg cgtggggggt ggcggcggag gaggcgagct gcggggagaa
      241 ggggcgacgg acggccgggc ttatggggct ggtggcggcg gtgcgggcag ggctggctgc
      301 tgcctgggca aggcagtccg aggtgccaaa ggtcaccacc atccccatcc cccaacctcg
      361 ggtgccgggg cggccggggg cgccgaggcg gatctgaagg cgctcacgca ctcggtgctc
      421 aagaaactca aggagcggca gctggagctg ctgcttcagg ccgtggagtc ccgcggcggt
      481 acgcgcaccg cgtgcctcct gctgcccggc cgcctggact gcaggctggg cccgggggcg
      541 cccgccagcg cgcagcccgc gcagccgccc tcgtcctact cgctccccct cctgctgtgc
      601 aaagtgttca ggtggccgga tctcaggcat tcctcggaag tcaagaggct gtgttgctgt
      661 gaatcttacg ggaagatcaa ccccgagctg gtgtgctgca acccccatca ccttagtcga
      721 ctctgtgaac tagagtctcc ccctcctcct tactccagat acccaatgga ttttctcaaa
      781 ccaactgcag gctgtccaga tgctgtacct tcctccgcgg aaaccgg
//
