LOCUS       sequence-157835-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Smad7 C terminal domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  TITLE     Moustakas plasmids
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Smad7-C
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             99..122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct tggtaccgag ctcggatcca ccgccatgga ctacaaggac gatgatgaca
      121 agggcgaatt cgggtctccc cctcctcctt actccagata cccaatggat tttctcaaac
      181 caactgcagg ctgtccagat gctgtacctt cctccgcgga aaccggggga acgaattatc
      241 tggcccctgg ggggctttca gattcccaac ttcttctgga gcctggggat cggtcacact
      301 ggtgcgtggt ggcatactgg gaggagaaga ctcgcgtggg gaggctctac tgtgtccaag
      361 agccctccct ggatatcttc tatgatctac ctcaggggaa tggcttttgc ctcggacagc
      421 tcaattcgga caacaagagt cagctggtac agaaagtgcg gagcaagatc ggctgtggca
      481 tccagctgac gcgggaagtg gatggcgtgt gggtttacaa ccgcagcagt taccccatct
      541 tcatcaagtc cgccacactg gacaacccgg actccaggac gctgttggtg cacaaagtgt
      601 tccctggttt ctccatcaag gcttttgact atgagaaagc ctacagcctg cagcggccca
      661 atgaccacga gttcatgcag caaccatgga cgggtttcac cgtgcagatc agctttgtga
      721 agggctgggg ccagtgctac acccgccagt tcatcagcag ctgcccgtgc tggctggagg
      781 tcatcttcaa cagccggtag tcgctcgagc atgcatctag agggccctat tctatagtgt
      841 cac
//
