LOCUS       sequence-157837-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Smad7 C terminal domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Moustakas plasmids
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Smad7-C
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(706..729)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(769..788)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(770..788)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggccctcta gatgcatgct cgagcgacta ccggctgttg aagatgacct ccagccagca
       61 cgggcagctg ctgatgaact ggcgggtgta gcactggccc cagcccttca caaagctgat
      121 ctgcacggtg aaacccgtcc atggttgctg catgaactcg tggtcattgg gccgctgcag
      181 gctgtaggct ttctcatagt caaaagcctt gatggagaaa ccagggaaca ctttgtgcac
      241 caacagcgtc ctggagtccg ggttgtccag tgtggcggac ttgatgaaga tggggtaact
      301 gctgcggttg taaacccaca cgccatccac ttcccgcgtc agctggatgc cacagccgat
      361 cttgctccgc actttctgta ccagctgact cttgttgtcc gaattgagct gtccgaggca
      421 aaagccattc ccctgaggta gatcatagaa gatatccagg gagggctctt ggacacagta
      481 gagcctcccc acgcgagtct tctcctccca gtatgccacc acgcaccagt gtgaccgatc
      541 cccaggctcc agaagaagtt gggaatctga aagcccccca ggggccagat aattcgttcc
      601 cccggtttcc gcggaggaag gtacagcatc tggacagcct gcagttggtt tgagaaaatc
      661 cattgggtat ctggagtaag gaggaggggg agacccgaat tcgcccttgt catcatcgtc
      721 cttgtagtcc atggcggtgg atccgagctc ggtaccaagc ttgggtctcc ctatagtgag
      781 tcgtattaat ttcgataagc cagtaagcag tgggttctct agttagccag agagctctgc
      841 ttatatagac ctc
//
