LOCUS       sequence-157838-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Smad7 delta C .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  TITLE     Moustakas plasmids
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Smad7-C-delta
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             87..110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(765..783)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(766..783)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(803..820)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttg
       61 gtaccgagct cggatccacc gccatggact acaaggacga tgatgacaag ggcgaattcg
      121 ggtctccccc tcctccttac tccagatacc caatggattt tctcaaacca actgcaggct
      181 gtccagatgc tgtaccttcc tccgcggaaa ccgggggaac gaattatctg gcccctgggg
      241 ggctttcaga ttcccaactt cttctggagc ctggggatcg gtcacactgg tgcgtggtgg
      301 catactggga ggagaagact cgcgtgggga ggctctactg tgtccaagag ccctccctgg
      361 atatcttcta tgatctacct caggggaatg gcttttgcct cggacagctc aattcggaca
      421 acaagagtca gctggtacag aaagtgcgga gcaagatcgg ctgtggcatc cagctgacgc
      481 gggaagtgga tggcgtgtgg gtttacaacc gcagcagtta ccccatcttc atcaagtccg
      541 ccacactgga caacccggac tccaggacgc tgttggtgca caaagtgttc cctggtttct
      601 ccatcaaggc ttttgactat gagaaagcct acagcctgca gcggcccaat gaccacgagt
      661 tcatgcagca accatggacg ggtttcaccg tgcagatcag ctttgtgaag ggctggggcc
      721 agtgctacac ccgctagctc gagcatgcat ctagagggcc ctattctata gtgtcaccta
      781 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgc
//
