LOCUS       sequence-157839-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Smad7 delta C .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     Moustakas plasmids
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Smad7-C-delta
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(652..675)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(715..734)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(716..734)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggccctcta gatgcatgct cgagctagcg ggtgtagcac tggccccagc ccttcacaaa
       61 gctgatctgc acggtgaaac ccgtccatgg ttgctgcatg aactcgtggt cattgggccg
      121 ctgcaggctg taggctttct catagtcaaa agccttgatg gagaaaccag ggaacacttt
      181 gtgcaccaac agcgtcctgg agtccgggtt gtccagtgtg gcggacttga tgaagatggg
      241 gtaactgctg cggttgtaaa cccacacgcc atccacttcc cgcgtcagct ggatgccaca
      301 gccgatcttg ctccgcactt tctgtaccag ctgactcttg ttgtccgaat tgagctgtcc
      361 gaggcaaaag ccattcccct gaggtagatc atagaagata tccagggagg gctcttggac
      421 acagtagagc ctccccacgc gagtcttctc ctcccagtat gccaccacgc accagtgtga
      481 ccgatcccca ggctccagaa gaagttggga atctgaaagc cccccagggg ccagataatt
      541 cgttcccccg gtttccgcgg aggaaggtac agcatctgga cagcctgcag ttggtttgag
      601 aaaatccatt gggtatctgg agtaaggagg agggggagac ccgaattcgc ccttgtcatc
      661 atcgtccttg tagtccatgg cggtggatcc gagctcggta ccaagcttgg gtctccctat
      721 agtgagtcgt attaatttcg ataagccagt aagcagtggg ttctctagtt agccagagag
      781 ctctgcttat atagacctcc caccgtacac
//
