LOCUS       sequence-157840-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Smad7 delta .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     Moustakas plasmids
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Smad7-delta
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct actagagaac ccactgtctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccaccg ccatggacta caaggacgat
      121 gatgacaagg gcgaattcgg gttcaggacc aaacgatctg cgctcgtccg gcgtctctgg
      181 aggagccgtg cgcccggcgg cgaggacgag gaggagggcg tggggggtgg cggcggagga
      241 ggcgagctgc ggggagaagg ggcgacggac ggccgggctt atggggctgg tggcggcggt
      301 gcgggcaggg ctggctgctg cctgggcaag gcagtccgag gtgccaaagg tcaccaccat
      361 ccccatcccc caacctcggg tgccggggcg gccgggggcg ccgaggcgga tctgaaggcg
      421 ctcacgcact cggtgctcaa gaaactcaag gagcggcagc tggagctgct gcttcaggcc
      481 gtggagtccc gcggcggtac gcgcaccgcg tgcctcctgc tgcccggccg cctggactgc
      541 aggctgggcc cgggggcgcc cgccagcgcg cagcccgcgc agccgccctc gtcctactcg
      601 ctccccctcc tgctgtgcaa agtgttcagg tggccggatc tcaggcattc ctcggaagtc
      661 aagaggctgt gttgctgtga atcttacggg aagatcaacc ccgagctggt gtgctgcaac
      721 ccccatcacc ttagtcgact ctgtgaacta gagtctcccc ctcctcctta ctccagatac
      781 ccaatggatt ttctcaaacc aactgcaggc tgtccagatg ctgtaccttc ctccgcgg
//
