LOCUS       sequence-157849-        7477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Tetsu O, McCormick F
  TITLE     Beta-catenin regulates expression of cyclin D1 in colon carcinoma 
            cells.
  JOURNAL   Nature. 1999 Apr 1;398(6726):422-6.
  PUBMED    10201372
REFERENCE   2  (bases 1 to 7477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1999-04-1"; volume: "398(6726)"; pages: "422-6"
COMMENT     Sequence Label: Myc-tagged delta N (dominant-negative form) hu...
FEATURES             Location/Qualifiers
     source          1..7477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    270..391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(281..300)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     335..354
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(565..1359)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(676..695)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1286..1305
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1426..1755)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1440..1575
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1494..1513)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     1740..1760
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(1769..2197)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1879..1900)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2091..2110
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2243..2467
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2456..2473
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        2493..2511
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2493..2510
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(4608..4637)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4659..4678)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4660..4678)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4723..4926)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4752..4772)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4927..5306
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5478..5497
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5572..5676
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5677..5745,5746..6537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5895..5914)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6708..7296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7197..7216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7450..7467
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
      241 agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac
      301 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      361 tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat
      421 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
      481 catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg
      541 tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
      601 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
      661 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
      721 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
      781 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
      841 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
      901 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
      961 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     1021 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     1081 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     1141 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
     1201 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     1261 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     1321 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     1381 ctcttgatca gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag
     1441 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc
     1501 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     1561 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     1621 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     1681 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     1741 ccctaactga cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca
     1801 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     1861 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     1921 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     1981 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     2041 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     2101 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     2161 acacccgccg cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag
     2221 ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg
     2281 tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac
     2341 ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca
     2401 ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa
     2461 ggcacagtcg aggctgatca gcgagctcta gcatttaggt gacactatag aatagggccc
     2521 tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt cgtcgacatc
     2581 gatggatccc tcgcaagctt tttttttttt tgttttttaa tcagtctttt aaaactaagc
     2641 tatccgaact acataacagt tatgtatata tatatatttt ttaaacgcta caaagacttt
     2701 aaacagctgt tgataaaaat tttggtagaa tcatgaggtt cttctctcat ctacatattt
     2761 aaaaggagaa attgaaataa gaatgggtca aatattgacc aatgaactcg ataaacatca
     2821 actaatgtag ccacatggca caaaattaag agagtacaaa acaaaacctt tcaaggtggg
     2881 ggtggggggc aaattaagaa aagtgaaaca aaaccataaa caaagtagcg gggttcacga
     2941 cgctaaagct attctaaaga cttggtgacg agcgacagcg gctggggctg ggtcccggcc
     3001 agggagctgt gggaatgtaa ggaagaagtc gacggctgtg caattgatga ggagggggcg
     3061 gcaggctgca aagcggctgg gggcaggtcc agggccccgt tgggacagag ggcggaggcc
     3121 ttgtgggtgg cctcagcgag caggagggcg ggtggcggag gcatcatgga caggtgggcc
     3181 agggggtcgg gcttcaggga cagcgacaga ggctgggtct gctcagtctg tgacttggcg
     3241 tctcggggag gggagcctag caggttcggg gaggggggag gcgaatctag taagcttcca
     3301 tctgaagagg gtgggctgag gcagctgcct tcaccttgta tgtagcgaac gcactttttt
     3361 tttctcctgc aagggccgca ccagttattc tgttgatcaa ggccaaagcg cgctcggcat
     3421 ttcttaggag cgctcaggtc tgtaatcgga ggaagtgaaa ggcaaggatt taggaaacat
     3481 tcgctgtgtt cattggtctc tcccggctgc ttgtcccttt tcctcttctt cttctttcca
     3541 tagttatccc gcgcggacca gccggggtac agttgcatat gaagctgtcg ctccttccgg
     3601 gccagctcgt agtatttcgc ttgctcttct ctggacagtg catgccacct ccgcccaagg
     3661 atctggttga tggccgcgct ttctttcaac gtgcactcag ctacgacctt tgctctcatt
     3721 tccttcatat acaacatgaa tgcattaaga ggtttcttta tgtggggctt cttcttttct
     3781 tcttcctttt tggagtcctg atgctttgaa ctatggagtg agccgacatc actctgggac
     3841 gattcctgtt tgactgttgg tgtgactatg gccggatgcg gaatgcccgt cgtgtgtagc
     3901 gtatgatgtg gtgggaccat atggggaggg aacctggaca tggaagcatt gacggtcaga
     3961 gctgtggggt aggggtgtct gaatcctcct gtcgtgattg ggtacactgg ttgaccttgc
     4021 tgtggtacta accatcctag cggatggggg atttgtccta cggtgccagg cgatagtggg
     4081 taatacgggg atatatctgg agggtgcgga ggccgtggga ttcctgtttt ggggtctacg
     4141 tcggctggta agtgtggagg tgggtttccc ggcgtgaagt gttcattgct gtacgtgata
     4201 agaggcgtga gggggtggac atggtgaggg tgctgcacca ctggcacttt gttagagaca
     4261 atgtgtgccg gtgatgggga ccgggcatcc ttgagggctt gtctactctg gaggctccct
     4321 gcctggacat caagcagtgg ccatttcatc tggagatagg ttcgggcggt gggcgagagc
     4381 gatccgttgg ggaggtaggg gctcgtcagg tcggggatca tgatgaaggg gtagccggga
     4441 tacggtggcc ccttaaagag ccctccatct tgcctcttgg ccgcttcttc caaactttcc
     4501 cgggatttgt ctcggaaact ttcggagcga ggcggaggcc gtctttccgc ctcggaatcg
     4561 gaggagctgt tttgattcgt catggtggaa ttcgagctcg gatccttcag gtcctcctcg
     4621 gagatcagct tctgctccat ggtaccaagc ttgggtctcc ctatagtgag tcgtattaat
     4681 ttcgataagc cagtaagcag tgggttctct agttagccag agagctctgc ttatatagac
     4741 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
     4801 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
     4861 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
     4921 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     4981 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     5041 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     5101 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     5161 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     5221 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
     5281 ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag
     5341 cgcaaaacgc ctaaccctaa gcagattctt catgcaattg tcggtcaagc cttgccttgt
     5401 tgtagcttaa attttgctcg cgcactactc agcgacctcc aacacacaag cagggagcag
     5461 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc ataggggatc
     5521 gggagatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5581 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5641 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5701 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5761 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5821 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5881 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5941 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6001 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6061 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6121 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6181 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6241 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6301 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6361 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6421 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6481 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6541 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6601 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6661 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6721 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6781 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6841 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6901 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6961 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7021 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7081 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7141 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7201 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7261 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7321 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7381 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7441 accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
