LOCUS       sequence-157855-        6906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA-Daxx in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Tang J, Agrawal T, Cheng Q, Qu L, Brewer MD, Chen J, Yang X
  TITLE     Phosphorylation of Daxx by ATM contributes to DNA damage-induced p53
            activation.
  JOURNAL   PLoS One. 2013;8(2):e55813. doi: 10.1371/journal.pone.0055813. Epub 
            2013 Feb 6.
  PUBMED    23405218
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(2):e55813. doi"; pages: " 
            10.1371/journal.pone.0055813. Epub 2013 Feb 6"
COMMENT     Sequence Label: HA-Daxx/pRK5
FEATURES             Location/Qualifiers
     source          1..6906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        250..629
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        630..833
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     784..804
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     830..854
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1064..1082
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1064..1081
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1095..1114
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1170..1196
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      3442..3451
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3455..3589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3492..3511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3546..3565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3658..4015
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3658..3849
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3681..3701)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3866..4001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4035..4052)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4035..4051)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4044..4066)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4264..4719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4351..4370)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4561..4582
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4736..4755)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4855..4877
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4915..4933)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5001..5105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5106..5174,5175..5966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5324..5343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6137..6725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6626..6645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6879..6896
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattaattcg
      241 agctcgcccg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      601 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      661 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      721 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      781 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
      841 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      901 gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca
      961 agagtgacgt aagtaccgcc tatagagtct ataggcccac ccccttggct tcgttagaac
     1021 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata
     1081 gaataacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcacct
     1141 cggttctatc gattgaattc accatgggat acccctacga cgtccccgac tacgccggat
     1201 ccgccaccgc taacagcatc atcgtgctgg atgatgatga cgaagatgaa gcagctgctc
     1261 agccagggcc ctcccaccca ctccccaatg cggcctcacc tggggcagaa gcccctagct
     1321 cctctgagcc tcatggggcc agaggaagca gtagttcggg cggcaagaaa tgctacaagc
     1381 tggagaatga gaagctgttc gaagagttcc ttgaactttg taagatgcag acagcagacc
     1441 accctgaggt ggtcccattc ctctataacc ggcagcaacg tgcccactct ctgtttttgg
     1501 cctcggcgga gttctgcaac atcctctcta gggtcctgtc tcgggcccgg agccggccag
     1561 ccaagctcta tgtctacatc aatgagctct gcactgttct caaggcccac tcagccaaaa
     1621 agaagctgaa cttggcccct gccgccacca cctccaatga gccctctggg aataaccctc
     1681 ccacacacct ctccttggac cccacaaatg ctgaaaacac tgcctctcag tctccaagga
     1741 cccgtggttc ccggcggcag atccagcgtt tggagcagtt gctggcgctc tatgtggcag
     1801 agatccggcg gctgcaggaa aaggagttgg atctctcaga attggatgac ccagactccg
     1861 catacctgca ggaggcacgg ttgaagcgta agctgatccg cctctttggg cgactatgtg
     1921 agctgaaaga ctgctcttca ctgaccggcc gtgtcataga gcagcgcatc ccctaccgtg
     1981 gcacccgcta cccagaggtt aacaggcgca ttgagcggct catcaacaag ccagggcctg
     2041 ataccttccc tgactatggg gatgtgcttc gggctgtaga gaaggcagct gcccgacaca
     2101 gccttggcct cccccgacag cagctccagc tcatggctca ggatgccttc cgagatgtgg
     2161 gcatcaggtt acaggagcga cgtcacctcg atctcatcta caactttggc tgccacctca
     2221 cagatgacta taggccaggc gttgaccctg cactatcaga tcctgtgttg gcccggcgcc
     2281 ttcgggaaaa ccggagtttg gccatgagtc ggctggatga ggtcatctcc aaatacgcaa
     2341 tgttgcaaga caaaagtgag gagggcgaga gaaaaaagag aagagctcgg ctccaaggca
     2401 cctcttccca ctctgcagac acccccgaag cctccttgga ttctggtgag ggccctagtg
     2461 gaatggcatc ccaggggtgc ccttctgcct ccagagctga gacagatgac gaagacgatg
     2521 aggagagtga tgaggaagag gaggaggagg aggaagaaga agaggaggag gccacagatt
     2581 ctgaagagga ggaggatctg gaacagatgc aggagggtca ggaggatgat gaagaggagg
     2641 acgaagagga agaagcagca gcaggtaaag atggagacaa gagccccatg tcctcactac
     2701 agatctccaa tgaaaagaac ctggaacctg gcaaacagat cagcagatct tcaggggagc
     2761 agcaaaacaa aggacgcata gtgtcaccat cgttactgtc agaagaaccc ctggccccct
     2821 ccagcataga tgctgaaagc aatggagaac agcctgagga gctgaccctg gaggaagaaa
     2881 gccctgtgtc tcagctcttt gagctagaga ttgaagcttt gcccctggat accccttcct
     2941 ctgtggagac ggacatttcc tcttccagga agcaatcaga ggagcccttc accactgtct
     3001 tagagaatgg agcaggcatg gtctcttcta cttccttcaa tggaggcgtc tctcctcaca
     3061 actggggaga ttctggtccc ccctgcaaaa aatctcggaa ggagaagaag caaacaggat
     3121 cagggccatt aggaaacagc tatgtggaaa ggcaaaggtc agtgcatgag aagaatggga
     3181 aaaagatatg taccctgccc agcccacctt cccccttggc ttccttggcc ccagttgctg
     3241 attcctccac gagggtggac tctcccagcc atggcctggt gaccagctcc ctctgcatcc
     3301 cttctccagc ccggctgtcc caaacccccc attcacagcc tcctcggcct ggtacttgca
     3361 agacaagtgt ggccacacaa tgcgatccag aagagatcat cgtgctttca gactctgatt
     3421 aggtcgacct gcagaagctt ggccgccatg gcccaacttg tttattgcag cttataatgg
     3481 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3541 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg ggaattaatt
     3601 cggcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
     3661 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     3721 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     3781 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3841 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3901 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3961 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4021 gttaacagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4081 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4141 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     4201 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     4261 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4321 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4381 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4441 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     4501 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4561 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     4621 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4681 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     4741 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     4801 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4861 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     4921 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4981 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5041 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5101 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5161 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5221 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5281 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5341 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5401 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5461 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5521 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5581 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5641 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5701 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5761 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5821 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5881 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5941 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6001 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6061 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6121 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6181 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6241 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6301 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6361 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6421 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6481 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6541 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6601 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6661 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6721 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6781 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6841 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6901 cggaag
//
