LOCUS       sequence-157861-        5395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria.
            Promoter is not regulated by acidic conditions. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Valdivia RH, Falkow S
  TITLE     Bacterial genetics by flow cytometry: rapid isolation of Salmonella 
            typhimurium acid-inducible promoters by differential fluorescence 
            induction.
  JOURNAL   Mol Microbiol. 1996 Oct . 22(2):367-78.
  PUBMED    8930920
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 1996 Oct . 22(2):367-78."
COMMENT     Sequence Label: pFPV25.1
FEATURES             Location/Qualifiers
     source          1..5395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            1303..1390
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             complement(2023..2736)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2063..2083)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     2678..2706
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(3404..3422)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3490..3594
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3595..3663,3664..4455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3813..3832)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4626..5214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5115..5134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5368..5385
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgctcaca gcccatggcc ttgagctgtc ggcctatggc ctgcaaagtc ctgtcgttct
       61 tcatcgggcc accaagcgca gccagatcga gccgtcctcg gttgtcagtg gcgtcaggtc
      121 gagcaagagc aacgatgcga tcagcagcac caccgtaggc atcatggaag ccagcatcac
      181 ggttagccat agcttccagt gccacccccg cgacgcgctc cgggcgctct gcgcggcgct
      241 gctcacctcg gcggctacct cccgcaactc tttggccagc tccacccatg ccgcccctgt
      301 ctggcgctgg gctttcagcc actccgccgc ctgcgcctcg ctggcctgct gggtctggct
      361 catgacctgc cgggcttcgt cggccagtgt cgccatgctc tgggccagcg gttcgatctg
      421 ctccgctaac tcgttgatgc ctctggattt cttcactctg tcgattgcgt tcatggtcta
      481 ttgcctcccg gtattcctgt aagtcgatga tctgggcgtt ggcggtgtcg atgttcaggg
      541 ccacgtctgc ccggtcggtg cggatgcccc ggccttccat ctccaccacg ttcggcccca
      601 ggtgaacacc gggcaggcgc tcgatgccct gcgcctcaag tgttctgtgg tcaatgcggg
      661 cgtcgtggcc agcccgctct aatgcccggt tggcatggtc ggcccatgcc tcgcgggtct
      721 gctcaagcca tgccttgggc ttgagcgctt cggtcttctg tgccccgccc ttctccgggg
      781 tcttgccgtt gtaccgcttg aaccactgag cggcgggccg ctcgatgccg tcattgatcc
      841 gctcggagat catcaggtgg cagtgcgggt tctcgccgcc accggcatgg atggccagcg
      901 tatacggcag gcgctcggca ccggtcaggt gctgggcgaa ctcggacgcc agcgccttct
      961 gctggtcgag ggtcagctcg accggcaggg caaattcgac ctccttgaac agccgcccat
     1021 tggcgcgttc atacaggtcg gcagcatccc agtagtcggc gggccgctcg acgaactccg
     1081 gcatgtgccc ggattcggcg tgcaagactt catccatgtc gcgggcatac ttgccttcgc
     1141 gctggatgta gtcggccttg gccctggccg attggccgcc cgacctgctg ccggttttcg
     1201 ccgtaaggtg ataaatcgcc atgctgcctc gctgttgctt ttgcttttcg gctccatgca
     1261 atggccctcg gagagcgcac cgcccgaagg gtggccgtta ggccagtttc tcgaagagaa
     1321 accggtaagt gccccctccc ctacaaagta gggtcgggat tgccgccgct gtgcctccat
     1381 gatagcctac gagacagcac attaacaatg gggtgtcaag atggttaagg ggagcaacaa
     1441 ggcggcggat cggctggcca agctcgaaga acaacgagcg cgaatcaatg ccgaaattca
     1501 gcgggtgcgg gcaagggaac agcagcaaga gcgcaagaac gaaacaaggc gcaaggtgct
     1561 ggtgggggcc atgattttgg ccaaggtgaa cagcagcgag tggccggagg atcggctcat
     1621 ggcggcaatg gatgcgtacc ttgaacgcga ccacgaccgc gccttgttcg gtctgccgcc
     1681 acgccagaag gatgagccgg gctgaatgat cgaccgagac aggccctgcg gggctgcaca
     1741 cgcgccccca cccttcgggt agggggaaag gccgctaaag cggctaaaag cgctagcagc
     1801 acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt
     1861 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
     1921 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
     1981 ctaccgcatt aaagcttgca tgcctgcagg tctggacatt tatttgtata gttcatccat
     2041 gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt ctctcttttc
     2101 gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg gtaaaaggac
     2161 agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa cgcttccatc
     2221 ttcaatgttg tgtctaattt tgaagttaac tttgattcca ttcttttgtt tgtctgccat
     2281 gatgtataca ttgtgtgagt tatagttgta ttccaatttg tgtccaagaa tgtttccatc
     2341 ttctttaaaa tcaatacctt ttaactcgat tctattaaca agggtatcac cttcaaactt
     2401 gacttcagca cgtgtcttgt agttcccgtc atctttgaaa aatatagttc tttcctgtac
     2461 ataaccttcg ggcatggcac tcttgaaaaa gtcatgctgt ttcatatgat ctgggtatct
     2521 cgcaaagcat tgaacaccat aaccgaaagt agtgacaagt gttggccatg gaacaggtag
     2581 ttttccagta gtgcaaataa atttaagggt aagttttccg tatgttgcat caccttcacc
     2641 ctctccactg acagaaaatt tgtgcccatt aacatcacca tctaattcaa caagaattgg
     2701 gacaactcca gtgaaaagtt cttctccttt actcatatgt atatctcctt cttaaatcta
     2761 gaggatctta acattttcag cgatacccgc tgcagccagg atggctttag aacgggtctt
     2821 gccgacaccg tagatcgaag ttaacgcgat cacggcgtgt ttctgatcag gaatgttaat
     2881 gcctgctata cgggccacta tgcactccta ctatttaata tgtacgtacc atgctgaaaa
     2941 gcccgttttc aggatactca aatggaaacg tacagacata caaaagattg gctggctaat
     3001 ctagccagct caacccaact ttgcaagaaa aatatgcgaa aaaatcagcc ttggcgctgt
     3061 ttatgcttcg gctcggcact gcaaatcaca cggatgacac catcacgctt aacgattttg
     3121 cagttacggc ataatttctt gacggaagca cgaactttca tttttactct ccgtaacttc
     3181 tcaggcgacc aattaacggc cgtagccttt caggttcgcc ttcttcaatg cagactcata
     3241 ctgactggac atcatcagag tttgcacttg agccataaag tccataatca cgacaacaac
     3301 gataagcagt gaggtcccac cgaagtagaa cggcactttc attgcatcac gcatgaactc
     3361 cgggatcccc gggtaccgag ctcgaattct tgaagacgaa agggcctcgt gatacgccta
     3421 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     3481 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3541 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3601 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3661 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3721 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3781 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     3841 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3901 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3961 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4021 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4081 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     4141 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4201 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4261 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4321 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4381 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4441 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4501 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4561 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4741 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     4801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4981 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5041 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5101 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5161 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5221 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5281 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     5341 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
