LOCUS       sequence-157864-        8734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Yuza Y, Glatt KA, Jiang J, Greulich H, Minami Y, Woo MS, Shimamura 
            T, Shapiro G, Lee JC, Ji H, Feng W, Chen TH, Yanagisawa H, Wong KK, 
            Meyerson M
  TITLE     Allele-dependent variation in the relative cellular potency of 
            distinct EGFR inhibitors.
  JOURNAL   Cancer Biol Ther. 2007 May . 6(5):661-7.
  PUBMED    17495523
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Biol
            Ther. 2007 May . 6(5):661-7."
COMMENT     Sequence Label: pBabe EGFR ASVinsertion
FEATURES             Location/Qualifiers
     source          1..8734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(810..1409)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(893..913)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1390..1409
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1419..1748)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1433..1568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1733..1753
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(1790..3427,3428..3496,3497..5359,
                     5360..5431))
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNI
                     GSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKE
                     YHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSIL
                     EKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDER
                     MHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNS
                     TVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGS
                     VQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSH
                     QISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             complement(5459..5875)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5475..5491)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5507..5529)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6076..6275
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             6340..6809
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        6840..6944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6945..7013,7014..7805)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7965..8553
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8454..8473
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8707..8724
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgatgcat ggggtcgtgc gctcctttcg
      781 gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag
      841 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
      901 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
      961 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
     1021 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
     1081 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
     1141 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
     1201 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
     1261 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
     1321 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
     1381 aggcgcaccg tgggcttgta ctcggtcatg gtaagctttt tgcaaaagcc taggcctcca
     1441 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     1501 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1561 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1621 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     1681 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     1741 tgacacacat tccacagggt cgaccactgt gctggcgaat tcctacatct catgctccaa
     1801 taaattcact gctttgtggc gcgaccctta ggtattctgc attttcagct gtggagccct
     1861 taaagatgcc atttggcttg gcttccttgg gaaagaagtc ctgctggtag tcagggttgt
     1921 ccaggctaat ttggtggctg cctttctggg cccagtgggc agggctgtcg aatgtgctgt
     1981 tgacacaggt gggctggaca gtgttgagat actcggggtt gcccactgca gtgctgtggg
     2041 ggtcctggta gtgtgggtct ctgctgggcg cggggttcag aggctgattg tgatagacag
     2101 gattctgcac agagccagcg ggccttttgg gaacggactg gtttatgtat tcaggcactg
     2161 ggaggaaggt gtcgtctatg ctgtcctcag tcaaggcgcc tgtggggtct gagctgtatc
     2221 gctgcaagaa gctgtcttcc ttgatgggac agctttgcag cccatttcta tcaatgcaag
     2281 ccacggtgga attgttgctg gttgcactca gagagctcag gaggggagtc cgtgacgtgg
     2341 aggggctgct gaagaagccc tgctgtggga tgaggtactc gtcggcatcc accacgtcgt
     2401 ccatgtcttc ttcatccatc agggcacggt agaagttgga gtctgtagga cttggcaaat
     2461 gcattctttc atccccctga atgacaaggt agcgctgggg gtctcgggcc attttggaga
     2521 attcgatgat caactcacgg aactttgggc gactatctgc gtctatcatc cagcacttga
     2581 ccatgatcat gtagacatcg atggtacata tgggtggctg agggaggcgt tctcctttct
     2641 ccaggatgga ggagatctcg ctggcaggga ttccgtcata tggcttggat ccaaaggtca
     2701 tcaactccca aacggtcacc ccgtagctcc agacatcact ctggtgggta tagattctgt
     2761 gtaaaattga ttccaatgcc atccacttga taggcacttt gcctccttct gcatggtatt
     2821 ctttctcttc cgcacccagc agtttggcca gcccaaaatc tgtgatcttg acatgctgcg
     2881 gtgttttcac cagtacgttc ctggctgcca ggtcgcggtg caccaagcga cggtcctcca
     2941 agtagttcat gccctttgcg atctgcacac accagttgag caggtactgg gagccaatat
     3001 tgtctttgtg ttcccggaca tagtccagga ggcagccgaa gggcatgagc tgcgtgatga
     3061 gttgcacggt ggaggtgagg cagatgccca gcaggcggca cacgtggggg ttgtctacag
     3121 aagccacgct ggccatcacg taggcttcat cgaggatttc cttgttggct ttcggagatg
     3181 ttgcttctct taattccttg atagcgacgg gaattttaac tttctcacct tctgggatcc
     3241 agagtccctt atacaccgtg ccgaacgcac cggagcccag cactttgatc tttttgaatt
     3301 cagtttcctt caagatcctc aagagagctt ggttgggagc ttctccactg ggtgtaagag
     3361 gctccacaag ctccctctcc tgcagcagcc tccgcagcgt gcgcttccga acgatgtggc
     3421 gccttcgcat gaagaggccg atccccaggg ccaccaccag cagcaagagg agggccccca
     3481 ccatcccagt ggcgatggac gggatcttag gcccattcgt tggacagcct tcaagacctg
     3541 gcccagtgca tccgtaggtg cagtttggat ggcacaggtg gcacacatgg ccggcgtctg
     3601 cgtacttcca gaccagggtg ttgttttctc ccatgactcc tgccgggcag gtcttgacgc
     3661 agtgggggcc gtcaatgtag tgggcacact ggatacagtt gtctggtccc cgtcctgtgc
     3721 aggtgatgtt catggcctga ggcaggcact ctgggtggca ctgtatgcac tcagagttct
     3781 ccacaaactc ccttggctca ccctccagaa ggttgcactt gtccacgcat tccctgcctc
     3841 ggctgacatt ccggcaagag acgcagtccc tgggctccgg gccccagcag ccctcggggg
     3901 agcacaaggc atggcagacc tggcctgtgg ccttgcagct gttttcacct ctgttgctta
     3961 taattttggt tttctgaccg gaggtcccaa acagtttttt ccagtttatt gtatttgcat
     4021 agcacaaatt tttgtttcct gaaattatca catctccatc acttatctcc ttgagggagc
     4081 gtaatcccaa ggatgttatg ttcaggctga cgactgcaag agaaaactga ccatgttgct
     4141 tggtcctgcc gcgtatgatt tctaggttct caaaggcatg gaggtccgtc ctgttttcag
     4201 gccaagcctg aatcagcaaa aaccctgtga tttcctttac ggttttcaga atatccagtt
     4261 cctgtggatc cagaggagga gtatgtgtga aggagtcacc cctaaatgcc accggcagga
     4321 tgtggagatc gccactgatg gaggtgcagt ttttgaagtg tttaatattc gtagcattta
     4381 tggagagtga gtctttaaat tcaccaatac ctattccgtt acacactttg cggcaaggcc
     4441 cttcgcactt cttacacttg cggacgccgt cttcctccat ctcatagctg tcggccccac
     4501 aggctcggac gcacgagccg tgatctgtca ccacataatt acggggacac ttcttcacgc
     4561 aggtggcacc aaagctgtat ttgccctcgg ggttcacatc catctggtac gtggtggggt
     4621 tgtagagcat gagtgggggg caggtgtcct tgcacgtggc ttcgtctcgg aatttgcggc
     4681 agaccaggca gtcgctctcc cgggggcctg tgcagcctgc agcacactgg ttgtggcagc
     4741 agtcactggg ggacttgcca cggcagcgcc cggagcactg ctgggcacag atgattttgg
     4801 tcagtttctg gcagttctcc tctcctgcac cccagcagct cccattggga cagcttggat
     4861 cacacttttg gcagctgccc aggtggttct ggaagtccat cgacatgttg ctgagaaagt
     4921 cactgctgac tatgtcccgc cactggatgc tctccacgtt gcacagggca gggttgttgc
     4981 tgaaccgcac ggcgccatgc aggatttcct gtaaatttct catgggcagc tccttcagtc
     5041 cggttttatt tgcatcatag ttagataaga ctgctaaggc ataggaattt tcgtagtaca
     5101 tatttcctct gatgatctgc aggttttcca aaggaattcg ctccactgtg ttgagggcaa
     5161 tgaggacata accagccacc tcctggatgg tctttaagaa ggaaagatca taattcctct
     5221 gcacataggt aatttccaaa ttcccaagga ccacctcaca gttattgaac atcctctgga
     5281 ggctgagaaa atgatcttca aaagtgccca actgcgtgag cttgttactc gtgccttggc
     5341 aaactttctt ttcctccaga gcccgactcg ccgggcagag cgcagccagc agcgccagga
     5401 gcgctgcccc ggccgtcccg gagggtcgca tgatgtacca ccacactggg atccggccgg
     5461 cgcctagaga aggagtgagg gctggataaa gggaggatcg aggcggggtc gaacgaggag
     5521 gttcaagggg gagagacggg gcggatggag gaagaggagg cggaggctta gggtgtacaa
     5581 agggcttgac ccagggaggg gggtcaaaag ccaaggcttc ccaggtcacg atgtagggga
     5641 cctggtctgg gtgtccatgc gggccaggtg aaaagacctt gatcttaacc tgggtgatga
     5701 ggtctcggtt aaaggtgccg tctcgcggcc atccgacgtt aaaggttggc cattctgcag
     5761 agcagaaggt aacccaacgt ctcttcttga catctaccga ctggttgtga gcgatccgct
     5821 cgacatcttt ccagtgacct aaggtcaaac ttaagggagt ggtaacagtc tggccctaat
     5881 tttcagacaa atacagaaac acagtcagac agagacaaca cagaacgatg ctgcagcgct
     5941 gcagcagaca agacgcgcgg cttcggttcc aaaccgaaag caaaaattca gacggaggcg
     6001 ggaactgttt taggttctcg tctcctacca gaaccacata tcctgacggg gtcggattcc
     6061 acatcgactc ccttcctcag gtcgggccac aaaaacggcc cccaaagtcc ctgggacgtc
     6121 tcccagggtt gcggccgggt gttcagaact cgtcagttcc accacgggtc cgccagatac
     6181 agagctagtt agctaactag taccgacgca ggcgcataaa atcagtcata gacactagac
     6241 aatcggacag acacagataa gttgctggcc agcttgcctc ccggtggtgg gtcggtggtc
     6301 cctgggcagg ggtctcccga tcccggacga gcccccaaat gaaagacccc cgctgacggg
     6361 tagtcaatca ctcagaggag accctcccaa ggaacagcga gaccacaagt cggatgcaac
     6421 tgcaagaggg tttattggat acacgggtac ccgggcgact cagtcaatcg gaggactggc
     6481 gccccgagtg aggggttgtg ggctctttta ttgagctcgg ggagcagaag cgcgcgaaca
     6541 gaagcgagaa gcgaactgat tggttagttc aaataaggca cagggtcatt tcaggtcctt
     6601 ggggcaccct ggaaacatct gatggttctc tagaaactgc tgagggctgg accgcatctg
     6661 gggaccatct gttcttggcc ctgagccggg gcaggaactg cttaccacag atatcctgtt
     6721 tggcccatat tcagctgttc catctgttct tggccctgag ccggggcagg aactgcttac
     6781 cacagatatc ctgtttggcc catattcagg ctgcaggtgg cacttttcgg ggaaatgtgc
     6841 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6901 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6961 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7021 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7081 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7141 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7201 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7261 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7321 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7381 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7441 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7501 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7561 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7621 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7681 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7741 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7801 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttgcgg ccgcaaaagg
     7861 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7921 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7981 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8041 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8101 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8161 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8221 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8281 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8341 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8401 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8461 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8521 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8581 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8641 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8701 gagcgcagcg agtcagtgag cgaggaagcg gaag
//
