LOCUS       sequence-157866-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector for split sfCherry screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pET_sfCherry(1-10)_32aalinker_sfCherry(11)
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atggaggagg acaacatggc catcatcaag gagttcatga gattcaaggt gcacatggag
       61 ggcagcgtga acggccacga gttcgagatc gagggcgagg gcgagggcca cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      181 atcctgagcc cccagttcat gtacggcagc aaggcctacg tgaagcaccc cgccgacatc
      241 cccgactacc tgaagctgag cttccccgag ggcttcacct gggagagagt gatgaacttc
      301 gaggacggcg gcgtggtgac cgtgacccag gacagcagcc tgcaggacgg cgagttcatc
      361 tacaaggtga agctgctggg caccaacttc cccagcgacg gccccgtgat gcagaagaag
      421 accatgggct gggaggccag caccgagaga atgtaccccg aggacggcgc cctgaagggc
      481 gagatcaacc agagactgaa gctgaaggac ggcggccact acgacgccga ggtgaagacc
      541 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtgga catcaagctg
      601 gacatcacca gccacaacga ggacgacgtt ggtggtggcg gatcagaagg aggcggtagc
      661 gggggccctg gttcgggagg ggaaggttct gctgggggag ggagcgctgg cggggggtct
      721 tacaccatcg tggagcagta cgagagagcc gagggcagac acagcaccta a
//
