LOCUS       sequence-157871-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 
            promoter, and tet-incucibile RPR1 promoter with NotI cloning site 
            adjacent to gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Smith JD, Suresh S, Schlecht U, Wu M, Wagih O, Peltz G, Davis RW, 
            Steinmetz LM, Parts L, St Onge RP
  TITLE     Quantitative CRISPR interference screens in yeast identify 
            chemical-genetic interactions and new rules for guide RNA design.
  JOURNAL   Genome Biol. 2016 Mar 8;17(1):45. doi: 10.1186/s13059-016-0900-9.
  PUBMED    26956608
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2016-03-8"; volume: "17(1):45. doi"; pages: " 
            10.1186/s13059-016-0900-9"
COMMENT     Sequence Label: pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atcactttac ttttatctaa tctagacatt ttaatatatt gtaatatgtg tgtttgtttg
       61 gattattaag aagaataatt acaaaaaaaa ttacaaagga aggtaattac aacagaatta
      121 agaaaggaca agaaggagga agagaatcag ttcattattt cttctttgtt atataacaaa
      181 cccaagtagc gatttggcca tacattaaaa gttgagaacc accctccctg gcaacagcca
      241 caactcgtta ccattgttca tcacgatcat gaaactcgct gtcagctgaa atttcacctc
      301 agtggatctc tctttttatt cttcatcgtt ccactaacct ttttccatca gctggcaggg
      361 aacggaaagt ggaatcccat ttagcgagct tcctcttttc ttcaagaaaa gacgaagctt
      421 gtgtgtgggt gcgcgcgcta gtatctttcc acattaagaa atataccata aaggttactt
      481 agacatcact atggctatac atatatatat atatgtaact tagcaccatc gcgcgtgcat
      541 cactgcatgt gttaaccgaa aagtttggcg aacacttcac cgacacggtc atttagatct
      601 gtcgtctgca ttgcacgtcc cttagcctta aatcctaggc gggagcattc tcgtgtaatt
      661 gtgcagcctg cgtagcaact caacatagcg tagtctaccc agtttttcaa gggtttatcg
      721 ttagaagatt ctcccttttc ttcctgctca caaatcttaa agtcatacat tgcacgacta
      781 cctgcaggaa agaacaacaa gagttcc
//
