LOCUS       sequence-157876-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for expression of kinase inactive PAK2 with a CFP 
            fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Whalley HJ, Porter AP, Diamantopoulou Z, White GR, Castaneda-Saucedo
            E, Malliri A
  TITLE     Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal 
            Pak and promote mitotic spindle formation.
  JOURNAL   Nat Commun. 2015 Jun 16;6:7437. doi: 10.1038/ncomms8437.
  PUBMED    26078008
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-06-16"; volume: "6:7437. doi"; pages: " 
            10.1038/ncomms8437"
COMMENT     Sequence Label: pRetro-XT-PAK2-CFP(puro)-T402A
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        16..330
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    24..42
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    60..78
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    95..113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    131..149
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    167..185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    202..220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    238..256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     303..327
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      354..363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(405..426)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(666..685)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctttcgtctt cactcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
       61 ccctatcagt gatagagaac gatgtcgagt ttactcccta tcagtgatag agaacgtatg
      121 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      181 agagaacgta tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc
      241 ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc
      301 agagctcgtt tagtgaaccg tcagatcgcc tggagaagga tccgcggccg ctcgccacca
      361 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      421 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      481 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      541 tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      601 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      661 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      721 tgaaccgcat cgagctgaag g
//
