LOCUS       sequence-157879-       10109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10109)
  TITLE     Tet-On Advanced vectors
REFERENCE   2  (bases 1 to 10109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVtight Hygro DEST (w769-1)
FEATURES             Location/Qualifiers
     source          1..10109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     125..144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     378..395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    512..533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(548..553,554..571,572..578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    586..602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     591..613
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     610..626
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     610..626
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     645..665
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        647..665
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        693..919
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             920..1100
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1147..1272
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1765..1998
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2183..2227
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2494..2611
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2633..2947
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2641..2659
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2677..2695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2712..2730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2748..2766
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2784..2802
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2819..2837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2855..2873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2920..2944
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3025..3149
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3174..3179,3180..3197,3198..3204)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3258..3917
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3324..3343)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4259..4564
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4443..4462
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4605..4729)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4760..5348
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4813..4833)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5231..5242)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5404..5903
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5439..5462)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5836..5855
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5921..6946
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5921..6946
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             7320..7553
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7625..7746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7662..7681)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7716..7735
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7786..7921
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7941..7960)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7942..7960)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7970..7987)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7970..7986)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7979..8001)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8128..8583
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8215..8234)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8425..8446
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8609..8713
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8714..8782,8783..9574)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8932..8951)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9745..10109,1..224)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
       61 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      301 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      361 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      421 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
      481 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      541 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      601 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
      661 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
      721 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
      781 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
      841 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
      901 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
      961 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     1021 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     1081 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     1141 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     1201 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     1261 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     1321 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     1381 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     1441 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     1501 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     1561 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     1621 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1681 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1741 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1801 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1861 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1921 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1981 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     2041 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     2101 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     2161 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     2221 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     2281 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     2341 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     2401 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     2461 attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
     2521 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2581 acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc tcgagtttac
     2641 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat
     2701 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     2761 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc
     2821 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     2881 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     2941 gatcgcctgg agaattcgag ctcggtaccc ggggatccac cggttcgtcg actagtccag
     3001 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg agaaacgtaa
     3061 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     3121 tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac
     3181 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
     3241 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     3301 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     3361 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     3421 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     3481 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     3541 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     3601 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     3661 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     3721 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     3781 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     3841 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     3901 ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa cagtatgcgt
     3961 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     4021 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     4081 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     4141 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     4201 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     4261 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     4321 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     4381 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     4441 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     4501 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     4561 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
     4621 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     4681 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt gatatccagc
     4741 acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4801 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4861 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4921 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4981 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5041 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5101 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     5161 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5221 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     5281 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5341 ctccccgcct ggaattctgc agatatccgg actagtgatc tctcgaggtt aacgaattct
     5401 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     5461 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     5521 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     5581 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     5641 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     5701 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     5761 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     5821 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     5881 cttcctcatc tccgggcctt tcgacctgca tcccgccacc atgaaaaagc ctgaactcac
     5941 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     6001 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     6061 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     6121 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     6181 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     6241 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     6301 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     6361 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     6421 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     6481 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     6541 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     6601 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     6661 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     6721 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     6781 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     6841 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     6901 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta gatgccgacc
     6961 gaacaagagc tgatttcgag aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa
     7021 atgcgagaga acggccttac gcttggtggc acagttctcg tccacagttc gctaagctcg
     7081 ctcggctggg tcgcgggagg gccggtcgca gtgattcagg cccttctgga ttgtgttggt
     7141 ccccagggca cgattgtcat gcccacgcac tcgggtgatc tgactgatcc cgcagattgg
     7201 agatcgccgc ccgtgcctgc cgattgggtg cagatccgtc gagggcccgg tacctttaag
     7261 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7321 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     7381 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7441 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7501 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7561 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7621 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7681 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7741 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     7801 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7861 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7921 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     7981 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     8041 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     8101 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     8161 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8221 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8281 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8341 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8401 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     8461 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8521 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     8581 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8641 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8701 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8761 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8821 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8881 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8941 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     9001 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     9061 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9121 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9181 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9241 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9301 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9361 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9421 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9481 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9541 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9601 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9661 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9721 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9781 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9841 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9901 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9961 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10021 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10081 acagcccagc ttggagcgaa cgacctaca
//
