LOCUS       sequence-157880-        9121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9121)
  TITLE     Tet-On Advanced vectors
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVtight Blast DEST (w762-1)
FEATURES             Location/Qualifiers
     source          1..9121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     125..144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     378..395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    512..533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(548..553,554..571,572..578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    586..602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     591..613
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     610..626
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     610..626
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     645..665
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        647..665
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        693..919
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             920..1100
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1147..1272
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1765..1998
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2183..2227
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2494..2611
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2633..2947
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2641..2659
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2677..2695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2712..2730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2748..2766
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2784..2802
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2819..2837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2855..2873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2920..2944
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3025..3149
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3174..3179,3180..3197,3198..3204)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3258..3917
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3324..3343)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4259..4564
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4443..4462
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4605..4729)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4760..5348
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4813..4833)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5231..5242)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5399..5419)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5404..5733
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5584..5719
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5781..5828
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5847..6245
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6332..6565
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6637..6758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6674..6693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6728..6747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6798..6933
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6953..6972)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6954..6972)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6982..6999)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6982..6998)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6991..7013)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7140..7595
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7227..7246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7437..7458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7621..7725
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7726..7794,7795..8586)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7944..7963)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8757..9121,1..224)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
       61 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      301 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      361 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      421 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
      481 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      541 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      601 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
      661 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
      721 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
      781 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
      841 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
      901 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
      961 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     1021 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     1081 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     1141 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     1201 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     1261 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     1321 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     1381 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     1441 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     1501 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     1561 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     1621 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1681 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1741 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1801 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1861 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1921 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1981 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     2041 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     2101 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     2161 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     2221 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     2281 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     2341 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     2401 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     2461 attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
     2521 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2581 acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc tcgagtttac
     2641 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat
     2701 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     2761 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc
     2821 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     2881 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     2941 gatcgcctgg agaattcgag ctcggtaccc ggggatccac cggttcgtcg actagtccag
     3001 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg agaaacgtaa
     3061 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     3121 tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac
     3181 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
     3241 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     3301 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     3361 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     3421 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     3481 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     3541 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     3601 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     3661 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     3721 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     3781 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     3841 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     3901 ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa cagtatgcgt
     3961 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     4021 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     4081 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     4141 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     4201 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     4261 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     4321 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     4381 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     4441 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     4501 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     4561 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
     4621 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     4681 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt gatatccagc
     4741 acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4801 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4861 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4921 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4981 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5041 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5101 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     5161 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5221 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     5281 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5341 ctccccgcct ggaattctgc agatatccgg ttagtaatga gtttggaatt aattctgtgg
     5401 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5461 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5521 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5581 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5641 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5701 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5761 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     5821 aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa gaatccaccc
     5881 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     5941 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     6001 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     6061 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     6121 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     6181 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     6241 gctaagcaca attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
     6301 cttagccact ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga
     6361 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     6421 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6481 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6541 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     6601 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     6661 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6721 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     6781 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6841 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6901 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     6961 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     7021 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     7081 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     7141 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7201 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7261 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     7321 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     7381 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     7441 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     7501 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     7561 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     7621 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7681 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7741 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7801 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7861 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7921 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     7981 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8041 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8101 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8161 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8221 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8281 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8341 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8401 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8461 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8521 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8581 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8641 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8701 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8761 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8821 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8881 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8941 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9001 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9061 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9121 a
//
