LOCUS       sequence-157891-         613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Force free control for Actin-cpstFRET-Actin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Guo J, Wang Y, Sachs F, Meng F
  TITLE     Actin stress in cell reprogramming.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Dec 9;111(49):E5252-61. doi: 
            10.1073/pnas.1411683111. Epub 2014 Nov 24.
  PUBMED    25422450
REFERENCE   2  (bases 1 to 613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1411683111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-12-9- 9"; volume: "111"; issue: "49"; pages: "E5252-61"
COMMENT     Sequence Label: cpstFRET-Actin
FEATURES             Location/Qualifiers
     source          1..613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..30
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(94..96,97..609)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(136..157)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(397..416)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
       61 gagctgtaca aggaattcgg cggcggtggc ggggtgagca agggcgagga gctgttcacc
      121 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      181 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      241 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag
      301 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      361 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      421 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      481 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacgccat cagcgacaac
      541 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
      601 aacatcgagg acg
//
