LOCUS       sequence-157895-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast drug counter-selectable selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pFA6a-FTKanAX
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(42..75)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgtacgctgc aggtcgacgg atccccgggt taattaagat ctaccgttcg tatagcatac
       61 attatacgaa gttatcctag tcatatgcct acaacaacta agaaaatggc tatcatgcgg
      121 aagctggtga gaagaacagc atcgggacaa gggaaggaag aacaaagaca aagaaaacaa
      181 aagaaagcaa ttgaaaacaa aacaaaacaa ttttcattcc ttctcttatc attccttttc
      241 ttttcttttc tctcattcaa cgcactccat cgtatccgta ttcctcttat tttttctctt
      301 tctctatatc catttctttc tctctaggtg tgtcctctct ctctcttcaa tttctctact
      361 ccgcattcca acgcatcctt cccccaacct cccatttcct ccttacggcc cgatagcgat
      421 cgtctttccc tcgctatcac tcgctaccgg cccctcctct gcaccgtaac ctcctacgta
      481 tttaccatat cataaagttt tttccgacgc ttatcgctga ccccctgtcg ccctcctatt
      541 ggcttccgga ttatcttctt gtccataagg tgatccatgc ttcctgaaga ttcccgaaat
      601 gtgtccactt tggcggggaa tcattccatc cacttctttc tctctcgctt tcctcattcg
      661 gcgctcccct tccgcgtctc attggtcttc cgctccgttt ttgctttgcc gatgttactt
      721 ggggagaggt gcgataatcc tttcgcaaaa actcggtttg acgcctccca tggtataaat
      781 agtgggtggt ggacaggtgc cttcgctttt cttttcttta agcaagagaa gcttccatgg
      841 aaactagtgc gttcgaccag gctgcgcgtt ctcgcggcca tagcaaccga cgtacggcgt
      901 tgcgccctcg ccggcagcaa gaagccacgg aagtccgc
//
