LOCUS       sequence-157907-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast drug counter-selectable selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pFA6a-FHyTkAX
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..73)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta attaagatct accgttcgta tagcatacat
       61 tatacgaagt tatcctagtc atatgcctac aacaactaag aaaatggcta tcatgcggaa
      121 gctggtgaga agaacagcat cgggacaagg gaaggaagaa caaagacaaa gaaaacaaaa
      181 gaaagcaatt gaaaacaaaa caaaacaatt ttcattcctt ctcttatcat tccttttctt
      241 ttcttttctc tcattcaacg cactccatcg tatccgtatt cctcttattt tttctctttc
      301 tctatatcca tttctttctc tctaggtgtg tcctctctct ctcttcaatt tctctactcc
      361 gcattccaac gcatccttcc cccaacctcc catttcctcc ttacggcccg atagcgatcg
      421 tctttccctc gctatcactc gctaccggcc cctcctctgc accgtaacct cctacgtatt
      481 taccatatca taaagttttt tccgacgctt atcgctgacc ccctgtcgcc ctcctattgg
      541 cttccggatt atcttcttgt ccataaggtg atccatgctt cctgaagatt cccgaaatgt
      601 gtccactttg gcggggaatc attccatcca cttctttctc tctcgctttc ctcattcggc
      661 gctccccttc cgcgtctcat tggtcttccg ctccgttttt gctttgccga tgttacttgg
      721 ggagaggtgc gataatcctt tcgcaaaaac tcggtttgac gcctcccatg gtataaatag
      781 tgggtggtgg acaggtgcct tcgcttttct tttctttaag caagagaagc ttacatgtcc
      841 atgggtaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
      901 gacagcgtct ccgacctgat g
//
