LOCUS       sequence-157911-        4958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad range bacterial fluorescence labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Barbier M, Damron FH
  TITLE     Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling.
  JOURNAL   PLoS One. 2016 Mar 3;11(3):e0146827. doi: 
            10.1371/journal.pone.0146827. eCollection 2016.
  PUBMED    26937640
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-3"; volume: "11(3):e0146827. doi"; pages: " 
            10.1371/journal.pone.0146827. eCollection 2016"
COMMENT     Sequence Label: pUCP30T-mKo1
FEATURES             Location/Qualifiers
     source          1..4958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      72..660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     561..580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            732..840
                     /note="incP origin of transfer"
     primer_bind     1090..1107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1224..1245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1260..1265,1266..1283,1284..1290)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1298..1314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1303..1325
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1322..1338
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1322..1338
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1370..2023
                     /codon_start=1
                     /product="Kusabira-Orange fluorescent protein (Karasawa et 
                     al., 2004)"
                     /label=Kusabira-Orange
                     /translation="VSVIKPEMKMKYFMDGSVNGHEFTVEGEGTGKPYEGHQEMTLRVT
                     MAKGGPMPFSFDLVSHTFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLQFEDGGFAA
                     VSAHISLRGNCFEHKSKFVGVNFPADGPVMQNQSSDWEPSTEKITTCDGVLKGDVTMYL
                     KLAGGGNHKCQFKTTYKAAKKILKMPQSHFIGHRLVRKTEGNITELVEDAVAHC"
     primer_bind     complement(2063..2080)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2063..2079)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2072..2094)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(2240..3073)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3087..3438
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     promoter        join(3479..3484,3485..3501,3502..3507)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3696..4229
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
       61 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      181 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
      241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacata
      721 agctagctta tcggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
      781 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
      841 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
      901 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
      961 tatgcagcgg aaagtatacc ttaaggaatc cccatgttct ttcctgcgtt atcccctgat
     1021 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     1081 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     1141 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1201 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     1261 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     1321 acaggaaaca gctatgacat gattacgaat tcggcgcgcc aagaaggagg tgagtgtgat
     1381 taaaccagag atgaagatga agtacttcat ggacggctcc gtcaatgggc atgagttcac
     1441 agttgaaggt gaaggcacag gcaaacctta cgagggacat caagagatga cactacgcgt
     1501 cacaatggcc aagggcgggc caatgccttt ctcgtttgac ttagtgtcac acacgttctg
     1561 ttacggccac agacctttta ctaaatatcc agaagagata ccagactatt tcaaacaagc
     1621 atttcctgaa ggcctgtcat gggaaaggtc gttgcagttc gaagatggtg ggttcgctgc
     1681 cgtcagtgcg catataagcc ttagaggaaa ctgcttcgag cacaaatcca aatttgttgg
     1741 ggttaacttt cctgccgatg gtcctgtgat gcaaaaccaa agttctgatt gggagccatc
     1801 aaccgagaaa attactacct gcgacggagt tctgaagggt gatgttacga tgtacctaaa
     1861 acttgcggga ggcggcaatc acaaatgcca attcaagact acttacaagg cggcaaaaaa
     1921 gattcttaaa atgccacaaa gccatttcat cgggcatcgc ctcgtcagga aaaccgaagg
     1981 caacattact gagctggtag aagatgcagt agctcattgc tgaggatcct ctagagtcga
     2041 cctgcaggca tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2101 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2161 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaaa ggcaggccgg
     2221 gccgtggtgg ccacggcctc taggccagat ccagcggcat ctgggttagt cgagcgcggg
     2281 ccgcttccca tgtctcacca gggcgagcct gtttcgcgat ctcagcatct gaaatcttcc
     2341 cggccttgcg cttcgctggg gccttaccca ccgccttggc gggcttcttc ggtccaaaac
     2401 tgaacaacag atgtgtgacc ttgcgcccgg tctttcgctg cgcccactcc acctgtagcg
     2461 ggctgtgctc gttgatctgc gtcacggctg gatcaagcac tcgcaacttg aagtccttga
     2521 tcgagggata ccggccttcc agttgaaacc actttcgcag ctggtcaatt tctatttcgc
     2581 gctggccgat gctgtcccat tgcatgagca gctcgtaaag cctgatcgcg tgggtgctgt
     2641 ccatcttggc cacgtcagcc aaggcgtatt tggtgaactg tttggtgagt tccgtcaggt
     2701 acggcagcat gtctttggtg aacctgagtt ctacacggcc ctcaccctcc cggtagatga
     2761 ttgtttgcac ccagccggta atcatcacac tcggtctttt ccccttgcca ttgggctctt
     2821 gggttaaccg gacttcccgc cgtttcaggc gcagggccgc ttctttgagc tggttgtagg
     2881 aagattcgat agggacaccc gccatcgtcg ctatgtcctc cgccgtcact gaatacatca
     2941 cttcatcggt gacaggctcg ctcctcttca cctggctaat acaggccaga acgatccgct
     3001 gttcctgaac actgaggcga tacgcggcct cgaccagggc attgcttttg taaaccattg
     3061 ggggtgaggc cacgttcgac attccttgtg tataagggga cactgtatct gcgtcccaca
     3121 atacaacaaa tccgtccctt tacaacaaca aatccgtccc ttcttaacaa caaatccgtc
     3181 ccttaatggc aacaaatccg tcccttttta aactctacag gccacggatt acgtggcctg
     3241 tagacgtcct aaaaggttta aaagggaaaa ggaagaaaag ggtggaaacg caaaaaacgc
     3301 accactacgt ggccccgttg gggccgcatt tgtgcccctg aaggggcggg ggaggcgtct
     3361 gggcaatccc cgttttacca gtcccctatc gccgcctgag agggcgcagg aagcgagtaa
     3421 tcagggtatc gaggcggatt cacccttggc gtccaaccag cggcaccagc ggcgcgaatt
     3481 gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct cacgcaactg
     3541 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     3601 ttatgactgt ttttttgtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     3661 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagc aacgatgtta
     3721 cgcagcaggg cagtcgccct aaaacaaagt taggtggctc aagtatgggc atcattcgca
     3781 catgtaggct cggccctgac caagtcaaat ccatgcgggc tgctcttgat cttttcggtc
     3841 gtgagttcgg agacgtagcc acctactccc aacatcagcc ggactccgat tacctcggga
     3901 acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt cgaccaagaa gcggttgttg
     3961 gcgctctcgc ggcttacgtt ctgcccaggt ttgagcagcc gcgtagtgag atctatatct
     4021 atgatctcgc agtctccggc gagcaccgga ggcagggcat tgccaccgcg ctcatcaatc
     4081 tcctcaagca tgaggccaac gcgcttggtg cttatgtgat ctacgtgcaa gcagattacg
     4141 gtgacgatcc cgcagtggct ctctatacaa agttgggcat acgggaagaa gtgatgcact
     4201 ttgatatcga cccaagtacc gccacctaac aattcgttca agccgagatc ggcttcccgg
     4261 ccgcggagtt gttcggtaaa ttgtcacaac gccgcggcca attcgactca ccagtcacag
     4321 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4381 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4441 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4501 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4561 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4621 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4681 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4741 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4801 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4861 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4921 aaaggatcta ggtgaagatc ctttttgata atctcatg
//
