LOCUS       sequence-157918-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c-Myc13
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..570
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="TTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFTL
                     REVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRRL
                     TVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTLC
                     GLDTALYDGTASDGEQALYMSMPCP"
     terminator      576..773
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(834..851)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtaccactc ttgacgacac ggcttaccgg taccgcacca gtgtcccggg ggacgccgag
       61 gccatcgagg cactggatgg gtccttcacc accgacaccg tcttccgcgt caccgccacc
      121 ggggacggct tcaccctgcg ggaggtgccg gtggacccgc ccctgaccaa ggtgttcccc
      181 gacgacgaat cggacgacga atcggacgac ggggaggacg gcgacccgga ctcccggacg
      241 ttcgtcgcgt acggggacga cggcgacctg gcgggcttcg tggtcgtctc gtactccggc
      301 tggaaccgcc ggctgaccgt cgaggacatc gaggtcgccc cggagcaccg ggggcacggg
      361 gtcgggcgcg cgttgatggg gctcgcgacg gagttcgccc gcgagcgggg cgccgggcac
      421 ctctggctgg aggtcaccaa cgtcaacgca ccggcgatcc acgcgtaccg gcggatgggg
      481 ttcaccctct gcggcctgga caccgccctg tacgacggca ccgcctcgga cggcgagcag
      541 gcgctctaca tgagcatgcc ctgcccctaa tcagtactga caataaaaag attcttgttt
      601 tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg
      661 ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg
      721 cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga
      781 ttcgatacta acgccgccat ccagtgttta aacgagctcg aattctaggc ttcctcgctc
      841 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      901 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      961 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctg
//
