LOCUS       sequence-157926-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HO endonuclease expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   McCusker J
  TITLE     McCusker Lab Unpublished Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHS3
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(253..708)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(390..411)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     602..621
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     834..856
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     849..865
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     872..891
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        872..890
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     923..939
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     923..939
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gcgtcatcgt atgtgaatgc tgggtcgcta tactggctgt cgattcgata ctaacgccgc
       61 catccagtgt cgaaaacgaa ctcgaattca tcgatgatat cagatccact agtggcctat
      121 gcaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
      181 aattatatca gttattaccc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
      241 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
      301 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
      361 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
      421 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
      481 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
      541 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
      601 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
      661 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
      721 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      781 ggcgaagggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      841 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattgga
      901 gctccaccgc ggtggcggcc gctctagaac tagtggatcc cccgggctgc aggaattcga
      961 cttgaagaac atcccaatga tgccagctgg
//
