LOCUS       sequence-157928-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an inducible short hairpin targeting human GATA6 sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: tet-pLKO shGATA6 puro
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             42..662
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(115..134)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(864..881)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ggcaaagaat tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa
       61 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
      121 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa
      181 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
      241 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta
      301 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag
      361 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
      421 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg
      481 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc
      541 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct
      601 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt
      661 ccgcgtacag cggctcccgg gagttctagg gatctgcccc tctccctccc ccccccctaa
      721 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
      781 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
      841 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
      901 gaag
//
