LOCUS       sequence-157932-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an inducible short hairpin targeting human GATA6 sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: tet-pLKO shGATA6 puro
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        111..205
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     122..141
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    159..177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    306..423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        472..955
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     901..919
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
       61 tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgca atatttgcat
      121 gtcgctatgt gttctgggaa atcaccataa acgtgaaatc cctatcagtg atagagactt
      181 ataagttccc tatcagtgat agagacaccg gcccagacca cttgctatga aactgcagtt
      241 tcatagcaag tggtctgggt ttttgaattc tcgacctcga gacaaatggc agtattcatc
      301 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
      361 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      421 tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga gggggttggg
      481 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
      541 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
      601 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
      661 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
      721 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
      781 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
      841 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
      901 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
      961 aatc
//
