LOCUS       sequence-157942-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses mouse Piezo1 with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Coste B, Mathur J, Schmidt M, Earley TJ, Ranade S, Petrus MJ, Dubin 
            AE, Patapoutian A
  TITLE     Piezo1 and Piezo2 are essential components of distinct mechanically 
            activated cation channels.
  JOURNAL   Science. 2010 Oct 1;330(6000):55-60. doi: 10.1126/science.1193270. 
            Epub 2010 Sep 2.
  PUBMED    20813920
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1193270"; journalName: "Science"; date: "2010-10-1-
            1"; volume: "330"; issue: "6000"; pages: "55-60"
COMMENT     Sequence Label: mPiezo1-IRES-eGFP
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(8..721,722..724,725..727))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(11..721,722..724,725..727))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(53..74)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     402..421
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     661..682
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gccggcctta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
       61 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
      121 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      181 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
      241 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
      301 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      361 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      421 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
      481 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
      541 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
      601 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
      661 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
      721 tcaccatggt tgtggccata ttatcatcgt gtttttcaaa ggaaaaccac gtccccgtgg
      781 ttcggggggc ctagacgttt ttttaacctc gactaa
//
