LOCUS       sequence-157944-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  enhanced SpCas9 mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     crispr/cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pX330-Flag-eSpCas9-D10A
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
       61 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
      121 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
      181 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
      241 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
      301 cctgatcaaa aagtaccctg cgctggaaag cgagttcgtg tacggcgact acaaggtgta
      361 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
      421 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
      481 gatccggaag gcgcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
      541 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
      601 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag
      661 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag
      721 ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
//
