LOCUS       sequence-157947-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression FnCpf1 nuclease and Fn crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE4497
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     358..377
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        452..556
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(775..794)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      181 cttgtggaaa ggacgaaaca ccgtaatttc tactgttgta gatggagacg ttcgaacgtc
      241 tccttttttg tacgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
      301 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
      361 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
      421 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      481 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      541 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      601 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      661 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      721 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      781 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      841 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      901 gacagtaag
//
