LOCUS       sequence-157951-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter driving Avi-tagged protein containing Cerulean 
            protein fused to the carboxy-terminal domain of avian Ran 
            GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-ubb-Avi-Cerulean-RanGAP
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     102..123
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(172..192)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(199..243)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     regulatory      249..258
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
       61 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      121 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccattc ctccctggaa
      181 gtacaggttc tctcctccct catgccactc gatcttctgg gcctcaaaga tgtcattcag
      241 gccaccagcc atggtggcgg atccctgtaa acaaattcaa agtaagatta gcaatcacac
      301 atttccattt aaaaaatact acaatgttct agccaataat gtatgttttg cacttctccc
      361 atgttaaacg agatagttca cccgaaaatg aaattttact ttgcatttgg tcacccttaa
      421 atgttacaaa cctttaaaat aatagaaaac tattgactat acttgtagag aaaaataaat
      481 actatccaaa acaatggctg acagtggact atagaatgca ttttacctgc agcaaaattg
      541 tccaaatgat cctatatgaa aatttagggt cttacctagt gaaatgatag ctcattttgc
      601 acaagctctg agatttggct ttgtgcatca tgaaatctaa ttatgtcatg catttccaaa
      661 gcattggcca ttttaaacaa actgcacatc tgtgcagttt acattgtttt aaaataaaaa
      721 ataaaaaatt aagaaataaa gaataataat gttccacaac attacattag gtagcagatt
      781 ggcagagaac caaagggtgg ggccagtaag gaaggacttc tacacagtaa ggaaacgctg
      841 aaattcaact taatcactca gtcatttaaa aataaatact tttatttttt ttatttttaa
      901 aggtctaatg gtttcattag ataagactgt tattccttga ctgggatcat ttagaaccat
      961 ttgaagccac atattgcatt ttggaatttt aa
//
