LOCUS       sequence-157960-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea 
            asigna virus peptide (2A), and membrane-tethered mCherry protein 
            (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-flk1-cytoBirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..1001)
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(39..1001)
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(1017..1043)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(1050..1076)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gttagaagac ttcccctgcc ctctcctgat ccgatatctt tttctgcact acgcagggat
       61 atttcaccgc ccatccaggg ttttattatt ccatcctgct caagtaataa agccccctgt
      121 ttgtctattc cgcgtgaaat gccaaatatt tctttatcac caatgataag tttcactggg
      181 cgattaataa aattatccag cttttcccag cgcgacagat aaggtgccaa tccttcttgt
      241 tcgaagagtt ccaacgcagc acgtaattca cgtattagca tggccgccaa cgtattacga
      301 tcgagattga tccccgcttc ctgcagcgtg atccacccct gattaacgac actctcttca
      361 acacggcgca ttgccatgtt gatcccggct ccaatgacta tttgcgccgc atcgccagtt
      421 ttgccagtca gctccaccag aatgcctgcc agcttgcgat cctgcagata gaggtcatta
      481 ggccatttaa cacgaacttt atctgcaccc agcttgcgta atacttccgc catcacgata
      541 ccgataacca gacttaaacc aatcgccgcc gccgggcctt gttccagacg ccagaacatc
      601 gacaaatata agtttgcgcc aaaaggcgaa aaccatttcc gaccccggcg accacggcca
      661 gcctgctggt attctgcaat gcaagcatcg cccgatttaa gctctccgat acgatcaaga
      721 aggtactgat tcgtggagtc aatcactggc agcacggcta cactaccgcc atccagctga
      781 cccaatatct gtttagcatt aagtaactgg ataggctcag gcaggctgta tcctttaccc
      841 ggaacggtaa agacatcaac gccccagtca cgcagtgtct gaatgtgttt attaatagcc
      901 gcccggctca ttcccagcgt ttcacccaac tgctcgccag agtgaaattc accgttcgct
      961 aacagggcaa tcaatttcag tggcacggtg ttatccttca taggagatct gcctggaagt
     1021 gaggcatagt cgggcacatc gtaacccggg aggctagcat aatcaggaac atcatacccg
     1081 ggcaaacttg cgtaatc
//
