LOCUS       sequence-157963-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea 
            asigna virus peptide (2A), and membrane-tethered mCherry protein 
            (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-myl7-cytoBirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..42
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             49..75
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gcaagtttgc ccgggtatga tgttcctgat tatgctagcc tcccgggtta cgatgtgccc
       61 gactatgcct cacttccagg cagatctcct atgaaggata acaccgtgcc actgaaattg
      121 attgccctgt tagcgaacgg tgaatttcac tctggcgagc agttgggtga aacgctggga
      181 atgagccggg cggctattaa taaacacatt cagacactgc gtgactgggg cgttgatgtc
      241 tttaccgttc cgggtaaagg atacagcctg cctgagccta tccagttact taatgctaaa
      301 cagatattgg gtcagctgga tggcggtagt gtagccgtgc tgccagtgat tgactccacg
      361 aatcagtacc ttcttgatcg tatcggagag cttaaatcgg gcgatgcttg cattgcagaa
      421 taccagcagg ctggccgtgg tcgccggggt cggaaatggt tttcgccttt tggcgcaaac
      481 ttatatttgt cgatgttctg gcgtctggaa caaggcccgg cggcggcgat tggtttaagt
      541 ctggttatcg gtatcgtgat ggcggaagta ttacgcaagc tgggtgcaga taaagttcgt
      601 gttaaatggc ctaatgacct ctatctgcag gatcgcaagc tggcaggcat tctggtggag
      661 ctgactggca aaactggcga tgcggcgcaa atagtcattg gagccgggat caacatggca
      721 atgcgccgtg ttgaagagag tgtcgttaat caggggtgga tcacgctgca ggaagcgggg
      781 atcaatctcg atcgtaatac gttggcggcc atgctaatac gtgaattacg tgctgcgttg
      841 gaactcttcg aacaagaagg attggcacct tatctgtcgc gctgggaaaa gctggataat
      901 tttattaatc gcccagtgaa acttatcatt ggtgataaag aaatatttgg cattt
//
