LOCUS       sequence-157964-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-myl7-NLS-BirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..43
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             50..76
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             92..112
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgcaagtttg cccgggtatg atgttcctga ttatgctagc ctcccgggtt acgatgtgcc
       61 cgactatgcc tcacttccag gcagatctcc tccaaaaaag aagagaaagg tacgatctat
      121 gaaggataac accgtgccac tgaaattgat tgccctgtta gcgaacggtg aatttcactc
      181 tggcgagcag ttgggtgaaa cgctgggaat gagccgggcg gctattaata aacacattca
      241 gacactgcgt gactggggcg ttgatgtctt taccgttccg ggtaaaggat acagcctgcc
      301 tgagcctatc cagttactta atgctaaaca gatattgggt cagctggatg gcggtagtgt
      361 agccgtgctg ccagtgattg actccacgaa tcagtacctt cttgatcgta tcggagagct
      421 taaatcgggc gatgcttgca ttgcagaata ccagcaggct ggccgtggtc gccggggtcg
      481 gaaatggttt tcgccttttg gcgcaaactt atatttgtcg atgttctggc gtctggaaca
      541 aggcccggcg gcggcgattg gtttaagtct ggttatcggt atcgtgatgg cggaagtatt
      601 acgcaagctg ggtgcagata aagttcgtgt taaatggcct aatgacctct atctgcagga
      661 tcgcaagctg gcaggcattc tggtggagct gactggcaaa actggcgatg cggcgcaaat
      721 agtcattgga gccgggatca acatggcaat gcgccgtgtt gaagagagtg tcgttaatca
      781 ggggtggatc acgctgcagg aagcggggat caatctcgat cgtaatacgt tggcggccat
      841 gctaatacgt gaattacgtg ctgcgttgga actcttcgaa caagaaggat tggcacctta
      901 tctgtcgcgc tgggaaaagc tggataattt tattaatcgc ccagtgaaac ttatcattgg
      961 t
//
