LOCUS       sequence-157971-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2 (RFP)
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(60..570)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(123..141)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(598..654)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(698..722)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(719..757)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    769..787
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    805..823
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    840..858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    876..894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    912..930
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    947..965
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cttcatgtgc atgttctcct tgatcagctc gctcatggtg gccgcgcgcg ggtgaattgc
       61 tggggagaga ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga
      121 ggcttgcaga atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac
      181 cccgcctccc gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg
      241 ctattggcca cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct
      301 gcgagggtac tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc
      361 aaggaacctt cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta
      421 gcggcgaaga tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg
      481 gcgccgctgc gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg
      541 ctgccttgga aaaggcgcaa ccccaacccc ggatcctgta caacgcgtct gcagcctagg
      601 accggggttt tcttccacgt ctcctgcttg ctttaacaga gagaagttcg tggctccgga
      661 accggtgtcg acgaattcgc tagccaattc tccaggcgat ctgacggttc actaaacgag
      721 ctctgcttat ataggcctcc caccgtacac gcctacctcg acatacgttc tctatcactg
      781 atagggagta aactcgacat acgttctcta tcactgatag ggataaactc gacatacgtt
      841 ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata gggagtaaac
      901 tcgacatacg ttctctatca ctgataggga gtaaactcga catcgttctc tatcactgat
      961 agggagtaaa ctcga
//
