LOCUS       sequence-157972-        6504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MMLV LTR plasmid pSIREN-RetroQ-BPTF-sh5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Mayes K, Elsayed Z, Alhazmi A, Waters M, Alkhatib SG, Roberts M, 
            Song C, Peterson K, Chan V, Ailaney N, Malapati P, Blevins T, Lisnic
            B, Dumur CI, Landry JW
  TITLE     BPTF inhibits NK cell activity and the abundance of natural 
            cytotoxicity receptor co-ligands.
  JOURNAL   Oncotarget. 2017 May 12;8(38):64344-64357. doi: 
            10.18632/oncotarget.17834. eCollection 2017 Sep 8.
  PUBMED    28969075
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.17834"; journalName: "Oncotarget"; date: 
            "2017-05-12- 12"; volume: "8"; issue: "38"; pages: "64344-64357"
COMMENT     Sequence Label: pSIREN-RetroQ-BPTF-sh5
FEATURES             Location/Qualifiers
     source          1..6504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..510
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(46..69)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     443..462
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             531..1130
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(531..550)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1027..1047
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1311..1736
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(1839..1861)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1997..2017)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2002..2331
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2182..2317
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2353..2372
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2453..2470)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2624..3212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2704..2723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3383..4174,4175..4243))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4006..4025
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4244..4348)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4416..4434
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        4534..4914
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4915..5118
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5069..5089
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5119..5294
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5357..5556
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5757..6173
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6103..6125
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6141..6157
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        6197..6445
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6197..6217
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6368..6387
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
       61 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
      121 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
      181 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
      241 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
      301 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
      361 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
      421 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
      481 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt
      541 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
      601 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
      661 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
      721 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
      781 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
      841 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
      901 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
      961 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     1021 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     1081 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
     1141 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcggcg tctcgagata
     1201 tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac
     1261 gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     1321 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     1381 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agggtcgacc
     1441 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     1501 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     1561 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
     1621 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
     1681 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg
     1741 ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa
     1801 gctggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     1861 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     1921 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     1981 gtgtagatcc ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     2041 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     2101 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     2161 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     2221 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     2281 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttact
     2341 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     2401 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     2461 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2521 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2581 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2641 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2701 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2761 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2821 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2881 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2941 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3001 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3061 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3121 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3181 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3241 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3301 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3361 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3421 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3481 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3541 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3601 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3661 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     3721 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3781 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3841 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3901 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3961 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     4021 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4081 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4141 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4201 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4261 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4321 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     4381 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     4441 gtcttcaaga attagcttgg ccattgcata cgttgtatcc atatcataat atgtacattt
     4501 atattggctc atgtccaaca ttaccgccat gttgacattg attattgact agttattaat
     4561 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     4621 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     4681 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     4741 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     4801 ctattgacgt caatgacggt aaatggcccg ctctggcatt atgcccagta catgacctta
     4861 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     4921 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     4981 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     5041 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     5101 gtctatataa gcagagctca ataaaagagc ccacaacccc tcactcggcg cgccagtctt
     5161 ccgatagact gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc
     5221 cgaatcgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccacgac
     5281 gggggtcttt catttggggg ctcgtccggg atttggagac ccctgcccag ggaccaccga
     5341 cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt
     5401 ctatgtttga tgttatgcgc ctgcgtctgt actagttagc taactagctc tgtatctggc
     5461 ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca
     5521 gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga
     5581 ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt
     5641 ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca
     5701 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc
     5761 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     5821 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     5881 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     5941 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     6001 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     6061 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     6121 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggaattg
     6181 aagatctggg caggaagagg gcctatttcc catgattcct tcatatttgc atatacgata
     6241 caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca
     6301 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
     6361 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
     6421 tatcttgtgg aaaggacgag gatccggcga aaaccaagag tacattcaag agatgtactc
     6481 ttggttttcg ccttttttac gcgt
//
