LOCUS       sequence-157974-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2 (Blast)
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(37..547)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(100..118)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(575..631)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        complement(672..986)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(675..699)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    746..764
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    782..800
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    817..835
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    853..871
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    889..907
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    924..942
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    960..978
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ttggccatgg tttagttccc gcgcgcgggt gaattgctgg ggagagaggt cggtgattcg
       61 gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc ttgcagaatg cggaacaccg
      121 cgcgggcagg aacagggccc acactaccgc cccacacccc gcctcccgca ccgccccttc
      181 ccggccgctg ctctcggcgc gccctgctga gcagccgcta ttggccacag cccatcgcgg
      241 tcggcgcgct gccattgctc cctggcgctg tccgtctgcg agggtactag tgagacgtgc
      301 ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag gaaccttccc gacttagggg
      361 cggagcagga agcgtcgccg gggggcccac aagggtagcg gcgaagatcc gggtgacgct
      421 gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg ccgctgcgtt tcccggaacc
      481 acgcccagag cagccgcgtc cctgcgcaaa cccagggctg ccttggaaaa ggcgcaaccc
      541 caaccccgga tcctgtacaa cgcgtctgca gcctaggacc ggggttttct tccacgtctc
      601 ctgcttgctt taacagagag aagttcgtgg ctccggaacc ggtgtcgacg aattcgctag
      661 ccaattctcc aggcgatctg acggttcact aaacgagctc tgcttatata ggcctcccac
      721 cgtacacgcc tacctcgaca tacgttctct atcactgata gggagtaaac tcgacatacg
      781 ttctctatca ctgataggga taaactcgac atacgttctc tatcactgat agggagtaaa
      841 ctcgacatac gttctctatc actgataggg agtaaactcg acatacgttc tctatcactg
      901 atagggagta aactcgacat cgttctctat cactgatagg gagtaaactc gacatacgtt
      961 ctctatcact gatagggagt aaactcgaca tatc
//
