LOCUS       sequence-157977-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             118..369
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     306..327
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             388..564
                     /codon_start=1
                     /product="""Zdark"" protein, derived from the Z subunit of 
                     protein A, that binds to the dark state of LOV2 and is 
                     released upon irradiation (Wang et al., 2016)"
                     /label=Zdk1
                     /translation="MVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANL
                     LAEAKKLNDAQAPK"
ORIGIN
        1 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
       61 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
      121 aagcagaaga acggcatcaa ggcaaacttc aagatccgcc acaacatcga ggacggcggc
      181 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      241 cccgacaacc actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc
      301 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      361 ctgtacaagg gttctggaag tggatctatg gtggataaca aattcaataa agaaaagacg
      421 cgtgccggtg cggaaatcca ttctctgcca aacctgaatg ttgagcagaa gtttgccttc
      481 atcgtgagcc tgtttgatga tccatctcag agcgccaatc tgctggccga agccaaaaaa
      541 ctgaacgatg cccaggcccc aaaaggatcc ggatctggtg gatctggtaa aatgggcatg
      601 actgaggacg acaagagaag ctgctgcttg ttagagattc aggagaccga ggccaagtac
      661 taccgcaccc tggaggacat tgagaagaac tacatgggtc ccttgcggct ggtgctgagc
      721 ccggcggata tggctgctgt cttcatcaac ctggaggacc tcatcaaggt gcatcacagc
      781 tttctgcgag ccatcgatgt gtccatgatg gctggtggca gtaccctggc taaggtcttt
      841 ctggagttta aggaaaggct cctgatctat ggagagtact gtagccacat ggaacacgct
      901 cagagtacac tgaaccagct cctcgccagc cgagaggact tcaggcagaa agtggaggag
      961 tgcacactca gggttcagga t
//
