LOCUS       sequence-157978-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(51..74)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(81..113)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
ORIGIN
        1 accagccacc accttctgat aggcagcctg cacctgaggt taatcactta gtgatggtga
       61 tggtgatggt ggtgctcgag atcctcgggg tcttccgggg cgagttctgg ctggcttgca
      121 cgtgtataca gctgtgcggc cgcaagcttt tatgctccag gagcatccac atctgcaggt
      181 gaactcatct tgcagggggg gatcacctcc aggcattcct tgtgtgcccc gacgccacat
      241 ctggtacaca ggtatccctg gtagaaggta cccctgagaa acatcttgca ggctttgcag
      301 ttggtagtct tgtcgaatgt gtacatctgg aagctatgat ggttggcatt ggccttatct
      361 ggcttgatgt ttgacatggc catctcgaac tgctccatcc acttccgctt catgtcttcc
      421 gtcttgcaga agaactgaaa gccttgcttt ccttggaggt gaatcaggta gaagccatag
      481 gaccacttct tgatgtcctt gttgtgcatc gggtcatcgg tcatcttgtg gaagagcagc
      541 tcaatgacct ccttcagctc atagctgtag cccttcctct tgcacacgat gaccaccttg
      601 tcaaatagga acaggtacct gtcttgcttg gtgtggttga ctatggaccg gactttaagc
      661 tccccgtcaa tctttggcct cccaaattcc tccagcttca cttgcaggtt ttctatggag
      721 cactggaact cgctaatctt cttcaaggtc tccttgtccc gcttcacttc attgatgtac
      781 atggccaagt cctgcatggc ttccagggct tctttgagct gttgtctttc tggtcggtct
      841 gcagaatggc tcaggagctc cttgagcagc aggtggtact tcagcacccg ttgcatgggc
      901 accaccagca ggtcttgcag cttgaacttg ccatcctgaa ccctgagtgt gcactcctcc
      961 actt
//
