LOCUS       sequence-157979-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Zdk1 tagged Rac1 Q61L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mVenus-Zdk1-Rac1 Q61L
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    45..69
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        85..194
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             join(209..211,212..214,215..925)
                     /codon_start=1
                     /product="photostable derivative of mVenus (Lee et al., 
                     2020)"
                     /label=mGold
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(254..275)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(515..534)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     862..883
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcaaagaatt ggatcggacc gaaattaata cgactcacta taggggaatt gtgagcggat
       61 aacaattccc cggagttaat ccgggacctt taattcaacc caacacaata tattatagtt
      121 aaataagaat tattatcaaa tcatttgtat attaattaaa atactatact gtaaattaca
      181 ttttatttac aatcaaagga gatataccat ggtgagcaag ggcgaggagc tgttcaccgg
      241 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      301 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tcagcaccac
      361 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctcggctacg gcctgcagtg
      421 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      481 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      541 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      601 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      661 ctatatcacc gccgacaagc agaagaacgg catcaaggca aacttcaaga tccgccacaa
      721 catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      781 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccaaac tgagcaaaga
      841 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      901 tctcggcatg gacgagctgt acaagggttc tggaagtgga tctatggtgg ataacaaatt
      961 ca
//
