LOCUS       sequence-157983-        5165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Fcs under pBAD regulation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Lo TM, Chng SH, Teo WS, Cho HS, Chang MW
  TITLE     A Two-Layer Gene Circuit for Decoupling Cell Growth from Metabolite 
            Production.
  JOURNAL   Cell Syst. 2016 Aug;3(2):133-43. doi: 10.1016/j.cels.2016.07.012.
  PUBMED    27559924
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.07"; journalName: "Cell Syst"; date: "2016-08";
            volume: "3"; issue: "2"; pages: "133-43"
COMMENT     Sequence Label: pBbE8k-fcs
FEATURES             Location/Qualifiers
     source          1..5165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(7..885)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        912..1196
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1119..1138
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3162..3233
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3249..3276
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(3434..4022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3514..3533)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4110..4204)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4235..5029)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4346..4365)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4956..4975
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gacgtcttat gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa
       61 ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc
      121 aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt
      181 cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg
      241 gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc
      301 aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc
      361 catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag
      421 cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg
      481 cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt
      541 ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac
      601 ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg
      661 cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac
      721 cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat
      781 aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt
      841 aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc
      901 gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt
      961 ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca
     1021 aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa
     1081 agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat
     1141 aagattagcg gattctacct gacgcttttt atcgcaactc tctactgttt ctccataccc
     1201 gtttttttgg gaattcaaaa gatcttttaa gaaggagata tacatatgaa taacgaagcc
     1261 cgctcagggt cgaccgaccc tggccaacgt ccgcgctacc gccaggtggc catcgggcat
     1321 ccccaggtgc aggtcagtca cgtcgacgac gtgctgcgca tgcaacctgt cgagccactg
     1381 gcgccgctgc cggcgcgcct gctcgagcgc ctggtgcatt gggcccaggt gcgcccggac
     1441 accactttca tcgcggcacg ccaggcagac ggtgcctggc gttcgatcag ctacgtgcag
     1501 atgctcgccg atgtgcgcac catcgccgcc aacttgctag gactgggcct cagtgccgag
     1561 cgcccgctgg cgctgctttc cggcaacgac atcgaacacc tgcaaatcgc cctcggcgcc
     1621 atgtatgccg gtattgccta ttgcccggtg tcgccggcct acgcgctgtt gtcgcaagac
     1681 ttcgccaagt tgcgccatgt ctgcgaggtg ctcacccccg gagtggtctt cgtcagcgac
     1741 agccagccgt tccagcgcgc cttcgaggcg gtgctggacg attcggtcgg cgtgatcagc
     1801 gtgcgtggcc aggtcgcagg tcgcccccat ataagcttcg acagcctgtt gcaaccgggt
     1861 gacctggcgg cggccgatgc ggctttcgcc gccaccgggc cggacaccat cgccaaattc
     1921 ctcttcacct cgggctcgac caagctgccc aaggcggtga tcaccaccca gcgcatgctg
     1981 tgcgccaatc agcagatgct tctgcagact tttccgacgt tcgccgagga gccgccggtg
     2041 ctggtggact ggctgccgtg gaaccacacg ttcggcggta gccacaacct cggcatcgtg
     2101 ctttacaacg ggggcagttt ctacctggac gccggcaagc cgaccccgca aggcttcgcc
     2161 gagaccttgc gcaatttgcg cgagatttcc cccacggcct acctcaccgt acccaagggc
     2221 tgggaggaac tggtcaaggc actggagcag gaccccgcgc tacgcgaggt gttctttgcc
     2281 cgcatcaagc tgttcttctt tgccgccgca ggcctgtcgc aaagcgtctg ggaccggctg
     2341 gaccgcattg ccgagcaaca ctgtggcgaa cgcatccgca tgatggccgg ccttggcatg
     2401 accgaagcct cgccatcgtg caccttcacc accgggcctt tgtcgatggc cggctatgtc
     2461 gggctgccgg cacctggctg cgaagtgaag ctggtgccgg tgggcgacaa gctcgaggcg
     2521 cgcttccgtg gcccgcatat catgccgggc tactggcgct cgccgcagca gaccgccgag
     2581 gcgttcgacg aggagggctt ctactgttcg ggcgacgcgt tgaagctggc cgatgccagg
     2641 cagcccgagc ttggcctgat gttcgatggc cgtatcgctg aggacttcaa actttcgtcc
     2701 ggggtattcg tcagtgtcgg gccgctgcgc aaccgcgcag tgctggaggg ctcgccttac
     2761 gtacaggaca tcgtggtcac cgcgccggac cgtgaatgcc tgggcctgct ggtgttcccg
     2821 cgtctgcccg agtgtcggcg cctggccggg ctggcagagg atgccagcga tgcgcgggtg
     2881 ctggccaacg acaccgtgcg cagttggttc gctgactggc tggagcgctt gaaccgcgat
     2941 gcccaaggca acgccagccg tatcgaatgg ctgtcgctgc tggccgagcc gccgtcgatc
     3001 gacgccggtg aaatcaccga caagggctcg atcaatcagc gcgccgtgct gcagcggcgc
     3061 gccgctcagg tcgaggcgct gtaccgtggc gaagaccccg acgcattgca cgccaaggtg
     3121 cggccttaag gatccaaact cgagtaagga tctccaggca tcaaataaaa cgaaaggctc
     3181 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     3241 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacctagggc gttcggctgc
     3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4081 aagggatttt ggtcatgact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     4141 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     4201 agtccaagcg agctctcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     4261 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     4321 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     4381 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     4441 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     4501 gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     4561 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     4621 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     4681 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     4741 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     4801 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca
     4861 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     4921 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     4981 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     5041 ctcatcctgt ctcttgatca gatcatgatc ccctgcgcca tcagatcctt ggcggcaaga
     5101 aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca
     5161 attcc
//
