LOCUS       sequence-158007-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTFP1-Palladin-C-7
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..754
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg
       61 cgaggagacc acaatgggcg taatcaagcc cgacatgaag atcaagctga agatggaggg
      121 caacgtgaat ggccacgcct tcgtgatcga gggcgagggc gagggcaagc cctacgacgg
      181 caccaacacc atcaacctgg aggtgaagga gggagccccc ctgcccttct cctacgacat
      241 tctgaccacc gcgttcgcct acggcaacag ggccttcacc aagtaccccg acgacatccc
      301 caactacttc aagcagtcct tccccgaggg ctactcttgg gagcgcacca tgaccttcga
      361 ggacaagggc atcgtgaagg tgaagtccga catctccatg gaggaggact ccttcatcta
      421 cgagatacac ctcaagggcg agaacttccc ccccaacggc cccgtgatgc agaagaagac
      481 caccggctgg gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga
      541 cgtcaagcac aagctgctgc tggagggcgg cggccaccac cgcgttgact tcaagaccat
      601 ctacagggcc aagaaggcgg tgaagctgcc cgactatcac tttgtggacc accgcatcga
      661 gatcctgaac cacgacaagg actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg
      721 caactccacc gacggcatgg acgagctgta caagtccgga ctcagatctc gagtgagcgc
      781 tctggcctcc cgcgctaccg ccatgcagtc ctccggctcc ttcaactacg cgcgccctaa
      841 gcagttcatc gcg
//
