LOCUS       sequence-158024-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tet inducible bidirectional plasmid expressing GFP in one direction 
            and in the other a human DMPK genomic segment containing exons 11-15
            with 12 CTG repeats in exon 15 located.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Lee JE, Bennett CF, Cooper TA
  TITLE     RNase H-mediated degradation of toxic RNA in myotonic dystrophy type
            1.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4221-6. doi: 
            10.1073/pnas.1117019109. Epub 2012 Feb 27.
  PUBMED    22371589
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1117019109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-03-13- 13"; volume: "109"; issue: "11"; pages: "4221-6"
COMMENT     Sequence Label: pBItetDT12nGFP
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcactcgac gcggccagct
       61 tggtcgagct ggatacttcc cgtccgccag ggggacatgc cggcgatgct gaaggtcgcg
      121 cgcattcccg atgaagaggc cggttactcg agcgcaggcg cagtggaagg aggatggccg
      181 cgcgcgctgc cagcccagcc ccctcttctc gacgctcggt ggcacagctg ggccacagct
      241 gggcgggggc ggtgcctccg ggtggcccgc tcgccctcct attggccgga cgccaaagcc
      301 ccgccccgtg gcttttcctc ccccaaccct gattcggccg cttcgcatcc cgctagctcc
      361 tcccagacct tcggccgcct ccacacgcct ccggattggc ccgctgcgga gcctccggcc
      421 cacaacgcaa accgcggaca ctgtggagtc cagagctttg ggcagatgga gggcctttta
      481 ttcgcgaggg tcgggggtgg gggtcctagg tggggacaga caatagatac cgaggaatgt
      541 cggggtctca gtgcatccaa aacgtggatt ggggttgttg ggggtcctgt agcctgtcag
      601 cgagtcggag gacgaggtca ataaatatcc aaaccgccga agcgggcggg gccggctggg
      661 gctccgagag cagcgcaagt gaggaggggg gcgcgggatc cccgaaa
//
